Anti-Integrin β1 Antibody, clone N29

半岛bd体育手机客户端 说明

特异性

Reacts with the human integrin beta1 subunit. Specificity verified by preclearing of beta1in immunoprecipitation, flow cytometry on transfectant cells displaying human beta1 integrin, and reactivity with purified beta1. Recognizes an epitope cluster distinct from MAB2250 and MAB2251(Wilkins et al., 1996).

免疫原

Jurkat T-leukemic cell line

应用

Research Category
Cell Structure
Research Sub Category
Integrins
This Anti-Integrin β1 Antibody, clone N29 is validated for use in FC, IH, IP, FUNC, CULT, WB for the detection of Integrin β1.
Immunohistochemistry (Paraffin) Analysis:A 1:50-2,000 dilution from a representative lot detected Integrin β1 in the membrane of tubule epithelial cells and cells of the glomeruli in human kidney tissue, membrane of alveolar cells, endothelial cells, and bronchiole epithelial cells of human lung tissue.

Immunoprecipitation:A representative lot of this antibody clone was used in immunoprecipitation.

Immunohistochemistry:A representative lot of this antibody clone was used in immunohistochemistry on frozen sections.

Flow Cytometry:A representative lot of this antibody clone was used in flow cytometry.

Functional Activity Assay:A representative lot of this antibody clone was used to stimulate adhesion of cells to extracellular matrix proteins (Wilkins, J.A. et al., 1996).

Optimal working dilutions must be determined by end user.

质量

Western Blot Analysis:0.5 ?g/mL of the antibody detected Integrin β1 in 10 ?g of U251 cell lysate.

目标描述

88 kDa

外形

Format: Purified
Protein A purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

储存及稳定性

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
Tonsil, human skin, human kidney tissue

U251 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	N29, monoclonal
species reactivity	human
manufacturer/tradename	Chemicon?
technique(s)	cell culture | mammalian: suitable flow cytometry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgGκ
NCBI登记号	NM_002292.3
UniProt登记号	P55268
运输	wet ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable