半岛bd体育手机客户端 说明
一般描述
Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.
特异性
MAB2247 recognizes the human beta1 integrin. This antibody has been shown to recognize a ligand-induced binding site, with antibody binding increasing in the presence of a fibronectin ligand. This antibody also enhances alpha 5 - beta 1 fibronectin interactions. MAB2247 does not cross-react with mouse or rat samples.
免疫原
Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract.
应用
Research Category
Cell Structure
Anti-Integrin β1 Antibody, clone 12G10 detects level of Integrin β1 & has been published & validated for use in ELISA, FC, IP, FUNC, CULT & WB.
Research Sub Category
Integrins
Flow cytometry: 1:25-1:50
ELISA : 10 μg/mL
Western Blot: known to react with human beta 1 integrin subunit under non-reduced conditions only {Mould, 1995}.
Stimulatory to alpha5beta1 interaction with fibronectin {Mould, 1995}.
Optimal working dilutions must be determined by end user.
目标描述
88 kDa
外形
Purified immunoglobulin. Liquid in phosphate buffer saline, with 0.1% sodium azide.
Format: Purified
Protein A purified
储存及稳定性
Maintain refrigerated at 2–8°C in undiluted aliquots for up one month. For long term storage maintain at -20°C for up to 12 months.
分析说明
Control
Tonsil, human skin tissue
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 12G10, monoclonal |
species reactivity | human |
manufacturer/tradename | Chemicon? |
technique(s) | ELISA: suitable cell culture | mammalian: suitable flow cytometry: suitable immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG1κ |
NCBI登记号 | NM_002211 |
UniProt登记号 | P05556 |
运输 | dry ice |
Gene Information | human ... ITGB1(3688) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich