Anti-Integrin alpha5 (Preservative Free) Antibody, clone SNAKA51

半岛bd体育手机客户端 说明

一般描述

Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The α5/β1 integrin, also termed VLA5 or CD49e, is identical to the fibronectin receptor and mediates the adhesion of cells to fibronectin.

免疫原

Whole cells corresponding to human Integrin alpha5.

应用

Research Category
Cell Structure
Activity Assay: A representative lot of this antibody converts alpha5 beta1 integrin into a ligand competant form and promotes fibronectin binding (Clark, K., et al. (2005). J Cell Sci. 118(Pt 2):291-300.).
Immunocytochemistry Analysis: A representative lot of this antibody detected Integin alpha 5 in Primary human foreskin fibroblasts (hFF) (Clark, K., et al. (2005). J Cell Sci. 118(Pt 2):291-300.; Valdembri, D., et al. (2009). PLoS Biol. 7(1):e25.).
Detect Integrin alpha5 (Preservative Free) using this Anti-Integrin alpha5 (Preservative Free) Antibody, clone SNAKA51 validated for use in Western Blotting, Activity Assay, ICC.

质量

Evaluated by Flow Cytometry in HeLa cell treated with fibronectin.
Flow Cytometry Analysis: 1 ?g of this antibody detected Integrin alpha5 in 0.5X10^6 HeLa cells.

目标描述

116 kDa calculated

外形

Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.
Protein G Purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.

分析说明

Control
HeLa cells

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	SNAKA51, monoclonal
纯化方式	using protein G
species reactivity	human
technique(s)	activity assay: suitable immunocytochemistry: suitable western blot: suitable
同位素/亚型	IgG2α
NCBI登记号	NP_001138472
UniProt登记号	P08648
运输	wet ice
Gene Information	human ... ITGAV(3685)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable