半岛bd体育手机客户端 说明

一般描述

Integrins are a family of dimeric proteins that mediate cell-cell and extracellular matrix adhesion. Each VLA subtype (VLA-1, VLA-2, VL-3, VLA-4, VLA-5) is composed of a distinct alpha subunit noncovalently associated with a common beta subunit, as an alpha-beta dimer. Integrin VLA-3 (very late antigen, alpha-3/beta-1) is a cell surface receptor that binds to fibronectin, laminin, collagen type I and VI. VLA-3 is typically restricted in normal tissue but is widely expressed in many cultured tumor cells and correlates with the degree of invasiveness of malignant melanoma cells.

特异性

Recognizes an epitope related to the VLA-3 complex (alpha-3/beta-1 integrin). Specificity was determined by immunoprecipitation from a detergent extract of³⁵S-methionine labeled KJ29 cells.

免疫原

Human renal carcinoma cell line (KJ29).

应用

Immunohistochemistry (works on Acetone-fixed paraffin-embedded tissues)

ELISA/RIA

Immunoprecipitation. When immunoprecipitated samples are run on SDS-PAGE under a) non-reducing conditions, two bands will be detected with an apparent molecular weight of 110 and 150kDa; and b) reducing (2% B-ME) conditions, a single molecular species of 130kDa is detected. Under reducing conditions both alpha3 and beta1 subunits run with the same apparent molecular weight.

Optimal working dilutions must be determined by end user.
Anti-Integrin α3β1 Antibody, clone M-KID2 detects level of Integrin α3β1 & has been published & validated for use in ELISA, IP, RIA, IC, IH(P).

外形

Immunoglobulin purified by Protein A. Purified immunoglobulin in PBS and 0.05% sodium azide as preservative.
Format: Purified

储存及稳定性

Maintain at 2°-8°C in undiluted aliquots for up to 12 months from date of receipt.

分析说明

Control
Cell line: KJ29 renal carcinoma adherent cells.
Tissue: Kidney; antibody reacts with basal layer podocytes and basement membrane of distal tubules.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	M-KID2, monoclonal
species reactivity	human
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable immunocytochemistry: suitable immunohistochemistry: suitable (paraffin) immunoprecipitation (IP): suitable radioimmunoassay: suitable
同位素/亚型	IgG1
NCBI登记号	NM_002204.1 NM_005501.1
UniProt登记号	P26006
运输	wet ice
Gene Information	human ... ITGA3(3675)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable