Anti-IDH1 Antibody, clone 12D6.1

半岛bd体育手机客户端 说明

一般描述

Isocitrate dehydrogenase [NADP] cytoplasmic (EC 1.1.1.42; UniProt O75874; also known as Cytosolic NADP-isocitrate dehydrogenase, IDH, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase) is encoded by the IDH1 (also known as PICD) gene (Gene ID 3417) in human. Isocitrate dehydrogenases (IDHs) catalyze the oxidative decarboxylation of isocitrate to produce alpha-ketoglutarate and carbon dioxide. There exist three forms of IDHs in human (IDH1-3). IDH3 utilizes NAD+ as a cofactor and functions within the citric acid cycle, while IDH1 and IDH2 utilize NADP+ as a cofactor and function outside the context of the citric acid cycle. Mutated IDH1 and IDH2 convert alpha-ketoglutarate to oncometabolite R(-)-2-hydroxyglutarate (2-HG) in cytosol and mitochondria, respectively. Isocitrate dehydrogenase 1 ? 2 mutations have been reported in gliomas, acute myeloid leukemias (AMLs), cartilaginous tumors, osteosarcoma, Giant cell tumors of bone (GCTB), Ollier disease, and Maffucci syndrome.

特异性

Clone 12D6.1 was raised against an internal sequence highly conserved among human, mouse and rat species (different by one amino acid), cross-reactivity to rat and murine species is likely, but has not been confirmed.

免疫原

KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human IDH1.

应用

This Anti-IDH1 Antibody, clone 12D6.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of IDH1.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected IDH1 in human prostate cancer tissue, as well as in normal kidney, pancreas, and testis tissue sections.

质量

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected IDH1 in 10 ?g of HepG2 cell lysate.

目标描述

~46 kDa observed. 46.53 kDa (Met1 removed) calculated.

外形

Protein G purified.
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	12D6.1, monoclonal
species reactivity	human
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
同位素/亚型	IgG2bκ
NCBI登记号	NP_001269315
UniProt登记号	O75874
运输	wet ice
Gene Information	human ... IDH1(3417)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable