Anti-Human IgG2 antibody, Mouse monoclonal

半岛bd体育手机客户端 说明

一般描述

Anti-Human IgG2 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the HP-6014 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgG2 myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads. Human IgG consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5, and 4% of the total IgG, respectively.

免疫原

Purified human IgG2 myeloma protein

应用

Anti-Human IgG2 antibody, Mouse monoclonal may be used in immunological techniques, including enzyme linked immunosorbent assay (ELISA), immunofluorescence, hemagglutination (HA) and hemagglutination inhibition.

生化/生理作用

Lipopolysaccharides stimulate an IgG2 response in peripheral blood leukocytes (PBL) and an IgG1 response in the spleen. Only IgG1 and IgG3 are capable of adherence to mononuclear phagocytes while IgG2 and IgG4 autoantibodies are much less efficient. The amount of the different IgG subclasses present in the blood shows variation with age. For example, IgG1 and IgG3 reach normal adult levels by 5-7 years of age while IgG2 and IgG4 levels raise more slowly, reaching adult levels at about 10 years of age. Serum IgG subclass deficiencies have been recorded for different patient groups. For example, IgG2 and IgG4 deficiency is found in patients of ataxia telangiectasia. Low IgG2 levels were found in patients with Systemic lupus erythematosus (SLE) and juvenile diabetes mellitus.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

基本信息

NACRES

NA.46

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
抗体形式	purified from hybridoma cell culture
antibody product type	primary antibodies
克隆	HP-6014, monoclonal
形式	buffered aqueous solution
species reactivity	human
浓度	~1.0 mg/mL
technique(s)	immunofluorescence: suitable indirect ELISA: 0.07-0.15 μg/mL using 1 μg/mL Human IgG2 myeloma for coating
同位素/亚型	IgG1
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable