Anti-Human IgE antibody, Mouse monoclonal

半岛bd体育手机客户端 说明

一般描述

Anti-Human IgE antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the GE-1 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified human IgE myeloma protein. Immunoglobulin E (IgE) is the least abundant immunoglobulin isotype in circulation. IgE is produced by plasma cells and is highly functional in the lung, skin and mucus membranes. Normal levels of IgE in serum are less than 1 μg/ml.

免疫原

purified human IgE myeloma protein

应用

Anti-Human IgE antibody, Mouse monoclonal has been used in various immunological techniques, including enzyme linked immunosorbent assay (ELISA), immunoblot, immunoprecipitation and IgE quantification using piezoelectric (Pz) crystal bioassay.

生化/生理作用

Immunoglobulin E (IgE) plays a critical role in allergic reactions and host protection from parasites. Serum IgE levels are highly elevated during an allergic reaction (such as atopic eczema, extrinsic asthma or hay fever, fungal and parasitic infection, atopic disease or primary immunodeficiency). Detection and measurement of total IgE concentrations is crucial for diagnosis, monitoring and treatment of allergy patients. In addition, the recognition and isolation of allergen-specific IgE can lead to progress in understanding of pathophysiologic mechanisms underlying IgE-associated allergic disorders.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

基本信息

NACRES

NA.46

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
抗体形式	purified from hybridoma cell culture
antibody product type	primary antibodies
克隆	GE-1, monoclonal
形式	buffered aqueous solution
species reactivity	human
浓度	~1.0 mg/mL
technique(s)	ELISA: 0.15-0.3 μg/mL using 1 μg/mL Human IgE myeloma for coating immunoblotting: suitable immunoprecipitation (IP): 1-2 μg/test using Human IgE myeloma
同位素/亚型	IgG2b
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
闪点(F)	Not applicable
闪点(C)	Not applicable