Anti-HMG-CoA reductase Antibody

半岛bd体育手机客户端 说明

一般描述

The 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase) enzyme is a ubiquitously expressed glycoprotein that is bound to the membrane of the endoplasmic reticulum (ER), but also projects an active C-terminal catalytic tail into the cytoplasm. It is the rate-limiting enzyme in the mevalonate pathway as it catalyzes the conversion of HMG CoA to mevalonate, which is a critical precursor protein in the synthesis of sterols such as cholesterols. In mammalian cells, HMG-CoA reductase is regulated by multiple mechanisms. It is downregulated by high levels of exogenous cholesterol bound to the low density lipoprotein. It is also regulated by sterol and nonsterol metabolites of mevalonate which may exert inhibitory effects at the transcriptional level. Previous studies have suggested that sterols may inhibit the sterol regulatory element-binding proteins (SREBPs) which function as transcription factors that enhance the expression of genes required for sterol biosynthesis. The end-stage degradation process may be mediated by the ubiquitin degradation system. The inhibition of HMG-CoA reductase has been widely studied for the treatment of cholesterol-related conditions.

特异性

Other homologies: Porcine (85% sequence homology).
This antibody recognizes HMG CoA reductase at the linker domain.

免疫原

Epitope: Linker domain
KLH-conjugated linear peptide corresponding to the linker domain of human HMG CoA reductase.

应用

Anti-HMG-CoA reductase Antibody detects level of HMG-CoA reductase & has been published & validated for use in Immunoprecipitation, Western Blotting.
Immunoprecipitation Analysis: 10 ?g/mL from a representative lot immunoprecipitated HMG CoA reductase from HepG2 cell lysate.

质量

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 1 ?g/mL of this antibody detected HMG CoA reductase on 10 ?g of HepG2 cell lysate.

目标描述

~97 kDa observed

联系

Replaces: 07-572

外形

Affinity purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	primate (based on 100% sequence homology)
technique(s)	immunoprecipitation (IP): suitable western blot: suitable
NCBI登记号	NP_000850
UniProt登记号	P04035
运输	wet ice
Gene Information	human ... HMGCR(3156)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable