Anti-Histone H3.3 Antibody, clone 4H2D7

半岛bd体育手机客户端 说明

一般描述

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

免疫原

KLH-conjugated linear peptide corresponding to human Histone H3.3.

应用

Research Sub Category
Histones
Immunocytochemistry Analysis: A 1:50 dilution from a represenative lot detected Histone H3.3 in HeLa cells.

Western Blotting Analysis: A representative lot from an independent laboratory detected recombinant Histone H3.3 (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Histone H3.3 in C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Histone H3.3 in C2C12 cells (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.3 from C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).
Research Category
Epigenetics & Nuclear Function
This Anti-Histone H3.3 Antibody, clone 4H2D7 is validated for use in western blotting, ICC & ChIP for the detection of Histone H3.3.

质量

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 2 ?g/mL of this antibody detected Histone H3.3 in 10 ?g of HeLa acid extract.

目标描述

~15 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

外形

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	rat
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	4H2D7, monoclonal
species reactivity	human, mouse
浓度	1 mg/mL
technique(s)	ChIP: suitable immunocytochemistry: suitable western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_002098
UniProt登记号	P84243
运输	wet ice
Gene Information	human ... H3F3B(3021)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable