Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5

半岛bd体育手机客户端 说明

一般描述

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.

Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

特异性

Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119.

免疫原

Recombinant mouse HP1a.

应用

Use Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5 (mouse monoclonal antibody) validated in ELISA, ICC, IHC to detect Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5.
Immunocytochemistry: 1:500-1:5,000

Immunohistochemistry: 1:500-1:5,000

ELISA: 1:500-1:5,000

Western blot: not recommended. For Western blot use MAB3446.

Optimal working dilutions must be determined by the end user.

外形

Unpurified
Liquid ascites no preservative.

分析说明

Control
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	2HP-1H5, monoclonal
species reactivity	human, mouse
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable immunocytochemistry: suitable immunohistochemistry: suitable
同位素/亚型	IgG1κ
适用性	not suitable for Western blot
NCBI登记号	NM_012117.1
UniProt登记号	P45973
运输	dry ice
Gene Information	human ... CBX5(23468)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable