Anti-Gs protein Antibody, alpha subunit, clone N192/12

半岛bd体育手机客户端 说明

一般描述

The heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) mediate signaling from a large number of G-protein coupled receptors (GPCR) to a variety of intracellular effectors. G-proteins are composed of a 36-52 kDa α-subunit, a 35-36 kDa β-subunit, and an 8-10 kDa γ-subunit. They are classified based on the nature of their α-subunit. Gs is ubiquitously expressed and couples receptors to adenylyl cyclase and promotes intracellular cAMP generation. Mutations in Gs-α can lead to constitutive activation of receptors leading to development of endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. In brain tissue Gs-α activates adenyl cyclase in direct response to stimulation of several neurotransmitter receptors and facilitates depolarization-induced neurotransmitter release for synaptic transmission. Also, it is shown that Gs-stimulated adenylyl cyclase activity is significantly reduced in the Alzheimer′s disease brain tissue.

特异性

Other homologies: Mouse (99% sequence homology).

免疫原

Recombinant protein corresponding to bovine GS protein, alpha subunit.

应用

Anti-Gs protein Antibody, alpha subunit, clone N192/12 is a highly specific mouse monoclonal antibody & that targets Gsα & has been tested in western blotting.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
Western Blotting Analysis: 2.0 ?g/mL from a representative lot detected GS protein, alpha subunit in 10 ?g of SH-SY5Y cell lysate.

质量

Evaluated by Western Blotting in rat brain membrane tissue lysate.

Western Blotting Analysis: 2.0 ?g/mL of this antibody detected GS protein, alpha subunit in 10 ?g of rat brain membrane tissue lysate.

目标描述

~45 kDa observed

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Rat brain membrane tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	N192/12, monoclonal
species reactivity	human, rat
species reactivity (predicted by homology)	bovine (immunogen homology)
technique(s)	western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_851364
UniProt登记号	P04896
运输	wet ice
Gene Information	human ... GNAS(2778)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable