Anti-GABA(A)R, Alpha1 Protein Antibody, clone N95/35

半岛bd体育手机客户端 说明

一般描述

The neurotransmitter GABA (gamma-aminobutyric acid) binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. Three general classes of GABA receptors are known: GABAA and GABAC (ionotropic) receptors and GABAB (metabotropic) receptors. The GABAA (gamma-Aminobutyric Acid type A) receptor is a multiprotein complex that acts as a ligand-gated chloride channel. These receptors are hetero-oligomers, for which 6 subunits are currently known (a, b, g, d, e and r). There are at least 6 different isoforms of the alpha subunit, and receptor subunit expression varies during development. Increased alpha1 subunit assembly in postsynaptic receptors of cerebellar inhibitory synapses is responsible for the fast inhibitory synaptic currents observed during postnatal development. Additionally, alpha1-containing GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 subunit increases upon chronic exposure to alcohol, making it a potential therapeutic target for alcohol dependency.

特异性

This antibody recognizes the cytoplasmic domain of GABA(A)R, Alpha1 Protein. This antibody does not demonstrate cross-reactivity against GABA(A)R2 and GABA(A)R3 (Prof. J. Trimmer, University of California, Davis).

免疫原

Recombinant protein corresponding to the cytoplasmic domain of mouse GABA(A)R, Alpha1 Protein.
Epitope: Cytoplasmic domain

应用

Immunohistochemistry Analysis: A representative lot detected GABA(A)R, Alpha1 Protein in rat hippocampus tissue (Prof. J. Trimmer, University of California, Davis).

Western Blot Analysis: A representative lot detected GABA(A)R, Alpha1 Protein in RBM membrane extracts, COS cell lysates transiently transfected with GABA(A)R, Alpha1 Protein, and in WT mice but not in GABA(A)R Alpha1 knockout mice.
Research Category
Neuroscience
This Anti-GABA(A)R, Alpha1 Protein Antibody, clone N95/35 is validated for use in Western Blotting, IHC for the detection of GABA(A)R, Alpha1 Protein, clone N95/35.
Research Sub Category
Neurotransmitters & Receptors

质量

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blot Analysis: 2 ?g/mL of this antibody detected GABA(A)R, Alpha1 Protein in 10 ?g of mouse brain tissue lysate.

目标描述

~55 kDa observed

外形

Purified mouse monoclonal IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Mouse brain tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	N95/35, monoclonal
species reactivity	rat, mouse
technique(s)	immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG2a
NCBI登记号	NP_034380
UniProt登记号	P62812
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable