Anti-FUS antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

Fused in sarcoma (FUS) is a component of heterogeneous nuclear ribonucleoprotein (hnRNP) complex is a DNA/ RNA binding protein. FUS gene is mapped to human chromosome 16p11.2 and is located predominantly in the nucleus.

特异性

Anti-FUS specifically recognizes human and rat FUS.

免疫原

synthetic peptide corresponding to the N-terminal region of human FUS isoform 1, conjugated to KLH. The corresponding sequence is identical in human FUS isoforms 2 and 3, and highly conserved (single amino acid insertion) in mouse and rat FUS.

应用

Anti-FUS antibody produced in rabbit has been used in:

• immunohistochemistry
• immunoblotting
• immunofluorescence

生化/生理作用

Fused in sarcoma (FUS) plays regulatory roles in transcription, RNA splicing and transport and is implicated in multiple diseases. FUS also called translocation in liposarcoma or Tumor lysis syndrome (TLS), plays a key role in DNA repair and transcriptional regulation. Chromosomal translocation of FUS/TLS is found in human cancers and results in the production of oncogenic FUS fusion proteins. FUS is a component of inclusion bodies in patients with Huntington′s disease (HD) and spinocerebellar ataxias (SCA1) and SCA3. Mutations in the FUS gene have been identified in amyotrophic lateral sclerosis (ALS), frontotemporal lobar degeneration (FLTD) and familial amyotrophic lateral sclerosis (FALS). The majority of the FUS mutations have been identified in the C-terminal nuclear localization signal (NLS). Pathological FUS inclusions are mostly found in the cytosol of neurons and glial cells.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

储存及稳定性

For continuous use, store at 2-8 ℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen ~68 kDa
species reactivity	rat, human
浓度	~1.0 mg/mL
technique(s)	immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin embedded rat colon. indirect immunofluorescence: 2.5-5 μg/mL using HeLa cells. western blot: 1.5-3.0 μg/mL using using lysates of Jurkat cells.
UniProt登记号	P35637
运输	dry ice
储存温度	?20℃
Gene Information	human ... FUS(2521) rat ... Fus(317385)

安全信息

储存分类代码	12 - Non Combustible Liquids
闪点(F)	Not applicable
闪点(C)	Not applicable