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Anti-Fox-3 Antibody

品牌
Sigma-Aldrich
货号
ABN51
规格纯度
from rabbit, purified by affinity chromatography
价格
5426.21 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-Fox-3 Antibody
RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Neuronal nuclei antigen, NeuN antigen
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

RNA binding protein fox-1 homolog 3 (UniProt: Q8BIF2; also known as Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Fox-3, Neuronal nuclei antigen, NeuN antigen) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID: 52897) in murine species. Fox-3 acts as a pre-mRNA alternative splicing regulator. It regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD). It is widely expressed in brain, including in cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, and in the spinal cord (at protein level). However, Purkinje cells lack Fox-3 expression. It is also found in the retina in the ganglion cells and some cells in the inner nuclear layer, but is absent from the photoreceptor cells and most cells in the inner nuclear layer. In developing mouse it is expressed in the neural tube as early as day E9.5. Fox-3 expression is shown to be up-regulated by retinoic acid in p19 cells during neural differentiation. Knockout mice display significantly reduced brain weight, impaired neurofilament expression and decreased white matter volume, but normal total body mass. Five isoforms of Fox-3 have been described that are generated by alternative splicing.

特异性

This antibody recognizes Fox-3 at the N-terminus.

免疫原

GST-tagged recombinant protein corresponding to the N-terminus of mouse Fox-3.
Epitope: N-terminus

应用

Western Blotting Analysis: 2 ?g/mL from a representative lot detected Fox-3 in mouse brain nuclear extract.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Fox-3 in mouse cerebral cortex, mouse hippocampus, and human cerebellum tissues.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Fox-3 in E18 rat cortex cells.

Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Anti-Fox-3, Cat. No. ABN51, is a highly specific rabbit polyclonal antibody that targets Fox 3/NeuN and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

质量

Evaluated by Western Blot in mouse e16 brain tissue lysate.

Western Blot Analysis: 0.5 ?g/mL of this antibody detected Fox-3 on 10 ?g of mouse e16 brain tissue lysate.

目标描述

~46 kDa observed. An uncharacterized band appears at ~68 kDa in some lysates.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Mouse e16 brain tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity mouse, rat, human
technique(s) immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI登记号 NP_001034256
UniProt登记号 Q8BIF2
运输 wet ice

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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