Anti-FIP200 Antibody, clone 14E11.2

半岛bd体育手机客户端 说明

一般描述

Focal adhesion kinase family interacting protein of 200 kDa (FIP200) is a novel protein inhibitor for focal adhesion kinase (FAK). FIP200 inhibits kinase activity and associated cellular functions, such as cell adhesion, spreading, and motility in fibroblasts by binding directly to FAK.

特异性

This antibody binds to the interaction domain of FIP200, which is needed for the formation of the ULK-FIP200 complex under starved, autophagy conditions.

免疫原

GST-tagged recombinant protein corresponding to the interaction domain of human FIP200.
Epitope: Interaction domain

应用

Western Blot Analysis: 1 ?g/mL from a representative lot detected FIP200 in serum starved and non-serum starved HeLa cell lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected FIP200 in NIH/3T3, A431, and HeLa cells.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected FIP200 in untreated and chloroquinone-treated HUVEC cells.

Research Sub Category
Apoptosis - Additional
Research Category
Apoptosis & Cancer
Anti-FIP200 Antibody, clone 14E11.2 is an antibody against FIP200 for use in Western Blotting, ICC.

质量

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 ?g/mL of this antibody detected FIP200 in 10 ?g of HeLa cell lysate.

目标描述

~200 kDa observed. An uncharacterized band may be observed at ~50 kDa in some cell lysates.

外形

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	14E11.2, monoclonal
species reactivity	human, mouse
technique(s)	immunocytochemistry: suitable western blot: suitable
同位素/亚型	IgG2bκ
NCBI登记号	NP_001077086
UniProt登记号	Q86YR4
运输	wet ice
Gene Information	human ... RB1CC1(9821)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable