Anti-Ferritin Heavy Chain Antibody, clone 1-2.3.1.2

半岛bd体育手机客户端 说明

一般描述

Ferritin is a widely expressed, very large, multi-subunit, intracellular protein that stores iron and releases it in a controlled fashion. The protein is produced by almost all living organisms in some form. In humans, it acts as a buffer against iron deficiency and iron overload. Ferritin protein is a multi-subunit (24 oligomers) protein complex consisting of both large (heavy) and small (light) chains. It is primarily and intracellular protein.The subunits of Ferritin in vertebrates have an apparent molecular weight of 19 kDa or 21 kDa respectively; their sequences are about 50% homologous. When Ferritin is unbound by iron the protein is called Apoferritin. Mutations associated with the Ferritin Heavy Chain protein contribute to a syndrome called Hemochromatosis 5 which is a disorder of iron metabolism where too much iron is retained within the tissues resulting in a variety of syndromes which unfortunately manifest themselves late in life.

免疫原

Recombinant protein corresponding to human Ferritin Heavy Chain.

应用

Research Sub Category
Apoptosis - Additional
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Ferritin Heavy Chain in human bone marrow and human tonsil tissue.
Immunohistochemistry Analysis: A representative lot detected Ferritin Heavy Chain in erythroid blasts of acute myeloid leukaemia M6 tissue, macrophages in the lymph node tissue, and tumor & lung-associated macrophages (Wang, W., et al. (2013). Histopahtology. 62:931–940).
This Anti-Ferritin Heavy Chain Antibody, clone 1-2.3.1.2 is validated for use in Western Blotting and Immunohistochemistry for the detection of Ferritin Heavy Chain.
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in U251 cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected Ferritin Heavy Chain in 10 ?g of U251 cell lysate.

目标描述

~21 kDa observed. Uncharacterized band may be observed at ~200 kDa

外形

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	1-2.3.1.2, monoclonal
species reactivity	human
technique(s)	immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_002023
UniProt登记号	P02794
运输	wet ice
Gene Information	human ... FTH1(2495)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable