Anti-FABP2 Antibody, clone 9A9B7B3

半岛bd体育手机客户端 说明

一般描述

Fatty acid-binding protein 2, (FABP2) is a fatty acid-binding protein produced by the intestinal tissues of the small intestine and participates in the transport and intracellular internalization and metabolism of fatty acids in the gut. Its expression is localized to the small intestine, especially within the cellular epithelial cells of the jejunum, with much lower levels in the large intestine. Its expression is confined to the cytoplasm and induced by growth factors such as EGF. FABP2 is also known as FABPI or I-FABP. Because of its role in fatty acid uptake and lipid homeostasis, mutations or polymorphisms affecting the function of FABP2 in fatty acid metabolism have been a source of recent research. In particular, an amino acid substitution known as A54T where the G to A polymorphism of codon 54 of the FABP2 gene results in the substation of a Thr in the mutated form instead of an Ala in the normal form. This Thr-codon 54 mutation demonstrates an increased affinity for long-chain fatty acids and is strongly associated with factors associated with metabolic syndrome disease, including insulin resistance, enhanced fatty acid oxidation, elevated triglycerides and type 2 diabetes. Furthermore, FABPs and in particular , FABP2, play an increasingly important role in controlling the lipid signaling cascade and FABP2 is being studied as key regulatory factor of lipid signaling under normal and pathological conditions. EMD-Millipore’s anti-FABP2 mouse monoclonal antibody, clone 9A9B7B3, has been tested in western blot, paraffin embedded immunohistochemical, and flow cytometry studies using human tissues and cell lines. The clone also reacts with FABP2 from NIH-3T3-L1 mouse cells lines by immunocytochemistry.

免疫原

Purified recombinant fragment of human FABP2 expressed in E. Coli.

应用

Detect Fatty Acid Binding Protein using this mouse monoclonal antibody, Anti-FABP2 Antibody, clone 9A9B7B3 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected FABP2 in 3T3-L1 cells.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected FABP2 in human small Intestine tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected FABP2 in LOVO cells.

Optimal working dilutions must be determined by end user.

质量

Evaluated by Western Blotting in human small intestine tissue lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected FABP2 in human small intestine tissue lysate.

目标描述

~15 kDa observed.

联系

Replaces: MABS390; MABS401

外形

Unpurified
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
Human small intestine tissue lysate

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	9A9B7B3, monoclonal
species reactivity	mouse, human
technique(s)	flow cytometry: suitable immunofluorescence: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG1
UniProt登记号	P12104
运输	wet ice
Gene Information	human ... FABP2(2169)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable