Anti-Exportin-1/CRM1 Antibody

半岛bd体育手机客户端 说明

一般描述

Exportin-1 (UniProt O14980; also known as Chromosome region maintenance 1 protein homolog, CRM1 homolog, Exp1) is encoded by the XPO1 (also known as CRM1) gene (Gene ID 7514) in human. The transport of macromolecules between cytoplasm and nucleus is mediated by importins and exportins and regulated by the small GTPase Ran. Most nuclear importins & exportins, including exportin-1, belong to the beta-karyopherin (KAP) family of proteins, Importins bind their cargoes with either nuclear localization signal (NLS) or M9 domain-containing hnRNP proteins in the cytoplasm, while exportins target their substrates via their nuclear export signal (NES) or M9 domain inside the nucleus. The energy dependence of nucleocytoplasmic transport replies on the Ran GTPase system, where the nucleotide exchange factor (NEF) RCC1/Prp20p, the GTPase activating protein (GAP) RanGAP1/Rna1p, and the Ran binding protein (RBP) RanBP1/Yrb1p are arranged asymmetrically to produce a high Ran GTP:Ran GDP ratio in the nucleus and the reverse ratio in the cytoplasm. Exportin 1/CRM1 is reported to bind RanGTP and either Rev or PKI NES peptides in a cooperative manner in vitro and exogenous expression of human CRM1 in Xenopus oocytes is shown to increase the rate of Rev and U snRNA nuclear export.

免疫原

Epitope: C-terminus
Recombinant protein corresponding to the C-terminus of human Exportin-1/CRM1.

应用

This Anti-Exportin-1/CRM1 Antibody is validated for use in Western Blotting, Immunocytochemistry, Immunoprecipitation, Electron Microscopy for the detection of Exportin-1/CRM1.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
Immunocytochemistry Analysis: Representative lots detected Crm1 nuclear localization in murine blastocysts and HeLa-derived HtTA-1 cells (Fornerod, M., et al. (1997). EMBO J. 16(4):807-816), as well as elevated nuclear envelope (NE) Crm1 accumulation in HeLa cells due to nuclear export inhibition upon oxidative stress induction by diethyl maleate (DEM) treatment (Crampton, N., et al. (2009). Mol Biol Cell. 20(24):5106-5116).
Immunoprecipitation Analysis: A representative lot detected enhanced Crm1 association with Nup62, Nup153, and Ran in HeLa cells upon diethyl maleate (DEM) treatment by co-IP (Crampton, N., et al. (2009). Mol Biol Cell. 20(24):5106-5116).
Western Blotting Analysis: A representative lot detected reduced Crm1 level in lysates from HeLa cells upon nuclear export inhibitor leptomycin B (LMB) or oxidant diethyl maleate (DEM) treatment (Crampton, N., et al. (2009). Mol Biol Cell. 20(24):5106-5116).
Electron Microscopy: A representative lot detected Crm1 nuclear localization in HeLa-derived HtTA-1 cells by EM (Fornerod, M., et al. (1997). EMBO J. 16(4):807-816).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Crm1 from COS-7 cell lysates (Boyle, S.M., et al.(1999). J Virol. 73(8):6872-6881).

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 of this antibody detected Exportin-1/CRM1 in 10 ?g of HeLa cell lysate.

目标描述

~120 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160702

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	serum
antibody product type	primary antibodies
克隆	polyclonal
species reactivity	mouse, human, monkey
technique(s)	electron microscopy: suitable immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable
NCBI登记号	NP_003391
UniProt登记号	O14980
运输	dry ice
Gene Information	human ... XPO1(7514)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable