半岛bd体育手机客户端 说明
一般描述
The activity of the main glutamate transporter in the CNS, Excitatory amino acid transporter 2 (also known as GLT1), can be regulated by protein kinase C (PKC). It is known that activation of PKC by phorbol esters promotes the clathrin-dependent internalization of the transporter, followed by its lysosomal degradation. However, the molecular mechanisms that link PKC activation and the internalization of the excitatory amino acid transporter 2 are not fully understood. The internalization process may play a role in the ubiquitylation of lysine residues located in the C-terminal tail of the excitatory amino acid transporter 2. Exposure to PMA increases the ubiquitylation of the excitatory amino acid transporter 2 in transfected cells and in the rat brain, and this ubiquitylated excitatory amino acid transporter 2 accumulates in the intracellular compartment. The activation of PKC induces the ubiquitylation of these C-terminal lysine residues in the Excitatory amino acid transporter 2 and this modification mediates the interaction of the transporter with the endocytic machinery.
特异性
This antibody recognizes the C-terminus of Excitatory amino acid transporter 2.
免疫原
Linear peptide corresponding to the C-terminus of human Excitatory amino acid transporter 2.
Epitope: C-terminus
应用
Western Blot Analysis: 0.5 ?g/ml from a previous lot detected Excitatory amino acid transporter 2 on 10 ?g of human brain tissue lysate.
Research Sub Category
Signaling Neuroscience
Research Category
Neuroscience
This Anti-Excitatory amino acid transporter 2 Antibody, clone G6 is validated for use in WB, IH, IC for the detection of Excitatory amino acid transporter 2.
质量
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 0.5 ?g/ml of this antibody detected Excitatory amino acid transporter 2 on 10 ?g of mouse brain tissue lysate.
目标描述
62 kDa
外形
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Mouse brain tissue lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | G6, monoclonal |
species reactivity | human, mouse |
species reactivity (predicted by homology) | rat (100% immunogen homology) |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG1κ |
NCBI登记号 | NP_004162 |
UniProt登记号 | P43004 |
运输 | wet ice |
Gene Information | human ... SLC1A2(6506) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich