Anti-Ero1 Antibody, clone 2G4/12

半岛bd体育手机客户端 说明

一般描述

ERO1-like protein alpha (ERO1) is also called endoplasmic oxidoreductin-1-like protein and Oxidoreductin-1-L-alpha. ERO1 is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. ERO1 is stimulated by hypoxia, indicating that it is regulated by the HIF-pathway. ERO1 may be responsible for much of the reactive oxygen species (ROS) in the cell, and therefore is a source of oxidative stress. EROS1 is required for the folding of immunoglobulin proteins, responsible for the release of the unfolded cholera toxin, and plays an important role in ER stress-induced, CHOP-dependent apoptosis. ERO1 is widely expressed at low levels, with high levels in upper digestive tract.

免疫原

GST-tagged recombinant protein corresponding to Human Ero1.

应用

Research Sub Category
Apoptosis - Additional
Western Blotting Analysis: 1 ?g/mL from a representative lot detected Ero1 from 10 ?g of the following cell lysates--A431, HEK293, HeLa, HepG2, human placenta tissue, HUVEC, jurkat, L6, NIH/3T3, PC12, PC3, and RAW264.7.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Ero1 in Ero1 transfected HeLa cell lysate (Prof. R. Sitia, San Rafaele Scientific Institute, Milano, Italy).

Immunofluorescence Analysis: A representative lot detected Ero1 in HeLa cell lysate (Prof. R. Sitia, San Rafaele Scientific Institute, Milano, Italy).
Anti-Ero1 Antibody, clone 2G4/12 is a highly specific mouse monoclonal antibody, that targets ERO1-like protein alpha & has been tested in western blotting, IP & Immunofluorescence.
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 ?g/mL of this antibody detected Ero1 in 10 ?g of HEK293 cell lysate.

目标描述

~56 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

外形

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HEK293 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	2G4/12, monoclonal
species reactivity	rat, mouse, human
technique(s)	immunofluorescence: suitable immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_055399
UniProt登记号	Q96HE7
运输	wet ice
Gene Information	human ... ERO1L(30001)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable