Anti-EMAPII Antibody, clone M7/1, (Preservat ive free)

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一般描述

Endothelial monocyte-activating polypeptide II (EMAPII) is a 22 kDa pro-inflammatory cytokine that regulates TNFβ signaling. The mature EMAPII is formed from a functional EMAPII precursor protein in response to apoptosis, and other forms of cell stress. Previous studies have reported that it may decrease the growth of tumors by inhibiting angiogenesis. EMAPII has also been implicated in autoimmune diseases. However, EMAPII is also involved in a range of normal biological processes. It is a component of the multisynthase complex that binds tRNA and plays an essential role in protein translation, and may also play a role in lung development.

特异性

This antibody recognizes the mature form of this protein at ~23 kDa and the larger pro EMAP II form at ~43 kDa which is secreted based on lysate.

免疫原

His-tagged recombinant protein corresponding to mouse EMAPII.

应用

Western Blot Analysis: A representative lot was used by an independent laboratory in recombinant EMAP II proteins. (Rajashekhar, G., et al. (2009). J Immunol Methods. 350(1-2):22–28.)

Neutralization Antibody: A representative lot was used by an independent laboratory to functionally neutralize pro and mature forms of EMAPII in lung tissue lysate. (Clauss, M., et al. (2012). J Clin Invest doi:10.1172/JCI43881.)

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in lung parenchyma of patients with emphysema. (Clauss, M., et al. (2012). J Clin Invest doi:10.1172/JCI43881.)
Anti-EMAPII Antibody, clone M7/1, (Preservat ive free) detects level of EMAPII & has been published & validated for use in Western Blotting, Activity Assay & IHC.
Research Category
Signaling
Research Sub Category
Developmental Signaling

质量

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected EMAPII in 10 ?g of HeLa cell lysate.

目标描述

~43 kDa observed. This antibody recognizes the mature form of this protein at ~23 kDa and the larger pro EMAP II form at ~43 kDa which is secreted based on lysate.

外形

Format: Purified
Purified rat monoclonal IgG2aκ in buffer containing PBS without preservatives.
Protein G Purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
HeLa cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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