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位置: 首页> 品牌> Sigma-Aldrich> Anti-EB1, EB2 and EB3 Antibody, clone KT65

Anti-EB1, EB2 and EB3 Antibody, clone KT65

品牌
Sigma-Aldrich
货号
MABT371
规格纯度
clone KT65, from rat
价格
5520.38 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
Anti-EB1, EB2 and EB3 Antibody, clone KT65
Microtubule-associated protein RP/EB family member 1, APC-binding protein EB1, End-binding protein 1, EB1, EB2, End binding protein 2, EB3, End binding protein 3, Microtubule-associated protein RP/EB family member 3
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

EB (End-binding) proteins are evolutionarily conserved and are important for extending tips of microtubules as well as regulating microtubule dynamics and communication with other intracellular substances. EB1 (End binding protein 1) encoded by the gene EB1/MAPRE1, EB2 (End binding protein 2) encoded by EB2/MAPRE2, and EB3 (End binding protein 3 also known as Microtubule-associated protein RP/EB family member 3) encoded by EB3/RP3/EBF3-S2/EBF3/MAPRE3 bind directly with end-tracking proteins (+TIPs). +TIPs influence microtubule growth, rescue, dynamicity, and suppression. EB1, EB2, and EB3 play major roles in cellular migration and adhesion, and are thought to associate with adenomatous polyposis coli tumor suppressor.

特异性

This antibody is expected to detect EB1, EB2 and EB3.

免疫原

GST-tagged recombinant protein corresponding to mouse EB1, EB2, and EB3.

应用

Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Immunocytochemistry Analysis: A representative lot detected EB1, EB2, and EB3 in NIH/3T3 cells (Van der Vaart, B., et al. (2011). J Cell Biol. 193(6):1083-1099).
Immunoprecipitation Analysis: A representative lot immunoprecipitated EB1, EB2, and EB3 in HeLa cell extracts (Van der Vaart, B., et al. (2011). J Cell Biol. 193(6):1083-1099).
This Anti-EB1, EB2 & EB3 Antibody, clone KT65 is validated for use in western blotting, ICC & IP for the detection of EB1.

质量

Evaluated by Western Blotting in MAPRE1 (EB1), MAPRE2 (EB2), and MAPRE3 (EB3) cell lysate.

Western Blotting Analysis: 1.0 ?g/mL of this antibody detected EB1, EB2, and EB3 in 10 ?g of MAPRE1 (EB1), MAPRE2 (EB2), and MAPRE3 (EB3) cell lysate.

目标描述

31, 37, 32 kDa calculated. The calculated MW of EB1 (31 kDa), EB2 (37 kDa), and EB3 (32 kDa). The recombinants used to test the reactivity of this antibody to EB1, EB2, and EB3 have the following MW′s. EB1 (56 kDa), EB2 (62 kDa), and EB3 (58 kDa).

外形

Purified rat monoclonal IgG2aκ in buffer containing PBS.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 rat
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 KT65, monoclonal
species reactivity mouse, human
technique(s) immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型 IgG2aκ
NCBI登记号 NP_031922
UniProt登记号 Q61166
Q6PER3
Q8R001
运输 wet ice
Gene Information human ... EBF3(253738), MAPRE2(10982), MAPRE3(22924)

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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