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Anti-E6AP Antibody

品牌
Sigma-Aldrich
货号
ABS989
规格纯度
serum, from rabbit
价格
5520.38 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-E6AP Antibody
Ubiquitin-protein ligase E3A, E6AP ubiquitin-protein ligase, Human papillomavirus E6-associated protein, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

E6AP/UB3A is an interesting E3 ubiquitin protein ligase with newly discovered alternative functions as well. It is the founding member of the HECT (Homologous to E6AP C terminus) family of ubiquitin ligases and recent research is shedding new light on its influence in health and disease. Ubiquitin substrates for E6AP/UB3A ligase include RAD23A/B, MCM7 (items involved in DNA replication), Annexin A1, PML tumor suppressor and the cell cycle regulator CDKN1B. E6AP also catalyzes the high risk human papilloma virus E6 mediated ubiquitination of p53, which contributes to the neoplastic progression of cells infected by these viruses. E6AP has also been identified as a steroid hormone receptor transcriptional coactivator, and E6AP has a role in numerous diseases including cancers and neurological syndromes. Additionally, E6AP/UB3A is developmentally important for adipogenesis, granulocyte maturation and synaptic processes, where it acts as a down-regulator. IE6AP/UBE3A protein is active as a trimer of E6AP subunits and widely expressed.

免疫原

a small portion of the full length protein expressed as a recombinant

应用

Research Category
Signaling
Research Sub Category
Signaling Neuroscience
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected E6AP in 10 ?g of A431, C2C12, L6, mouse brain, and PC3 cell lysate.
Western Blotting Analysis: A representative lot detected E6AP in HeLa cell lysate (Talis, A. L., et al. (1998). JBC. 273:6439-6445).
Immunoprecipitation Analysis: A representative lot immunoprecipitated E6AP in HeLa cell lysate (Wynn, H. K., et al. (2000). Journal of Virology. 74(14):6408-6417).
This Anti-E6AP Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of E6AP.

质量

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected E6AP in 10 ?g of A431 cell lysate.

目标描述

~100 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 serum
antibody product type primary antibodies
克隆 polyclonal
species reactivity rat, mouse, human
technique(s) immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型 IgG
NCBI登记号 NP_000453
UniProt登记号 Q05086
运输 wet ice
Gene Information human ... UBE3A(7337)

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 1

Sigma-Aldrich

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