Anti-Dnmt3b Antibody, clone D3B2-2C1

半岛bd体育手机客户端 说明

一般描述

DNA (cytosine-5)-methyltransferase 3B (Dnmt3B) is one of three mammalian enzymes that catalyze the addition of a methyl group to the 5’ carbon of cytosine residues in CpG dinucleotides. The Dnmt3b enzyme is involved in de novo methylation and the establishment of genome-wide DNA methylation patterns during development. DNA methylation has been linked to chromosome stability and gene expression in somatic cells; and to parental imprinting and X-chromosome inactivation in germ cells. Several studies have indicated that DNA methylation patterns are altered in various cancers.

免疫原

Linear peptide corresponding to human Dnmt3b.

应用

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate Dnmt3b in IP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19):8355-8365.).
Research Category
Epigenetics & Nuclear Function
Anti-Dnmt3b Antibody, clone D3B2-2C1 is a Rat Monoclonal Antibody for detection of Dnmt3b also known as DNA (cytosine-5)-methyltransferase 3B & has been validated in WB & IP.
Research Sub Category
Chromatin Biology

质量

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 0.5 ?g/mL of this antibody detected Dnmt3b in 10 ?g of of HeLa nuclear extract.

目标描述

~110 kDa observed. Multiple isoforms between ~80-96 kDa are known to exist. A truncated protein at ~40 kDa may be observed in some cell lysates (Ostler, K. R., et al. (2007). 26(38): 5553-5563.). Uncharacterized bands may be observed at ~20 kDa and ~34 kDa in some cell lysates.

外形

Format: Purified
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa nuclear extract

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	rat
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	D3B2-2C1, monoclonal
species reactivity	human
包装	antibody small pack of 25 μg
technique(s)	immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG2bκ
NCBI登记号	NP_001193984
UniProt登记号	Q9UBC3
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable