Anti-Dnmt1 Antibody, clone DNM-2C1

半岛bd体育手机客户端 说明

一般描述

DNA (cytosine-5)-methyltransferase 1 (Dnmt1) is one of three mammalian enzymes that catalyze the addition of a methyl group to the 5’ carbon of cytosine residues in CpG dinucleotides. These methylation marks are heritable modifications that are transferred to new DNA molecules following replication, by the activity of Dnmt1, and possibly Dnmt3a and Dnmt3b. Dnmt1 recognizes hemimethylated CpG regions with the aid of the UHRF1 protein, and it mediates the methylation of cytosines at those sites. DNA methylation has been linked to chromosome stability and gene expression in somatic cells; and to parental imprinting and X-chromosome inactivation in germ cells. Several studies have indicated that DNA methylation patterns are altered in various cancers.

免疫原

His-tagged recombinant protein corresponding to human Dnmt1.

应用

Anti-Dnmt1 Antibody, clone DNM-2C1 is a Rat Monoclonal Antibody for detection of Dnmt1 also known as DNA (cytosine-5)-methyltransferase 1 & has been validated in IP, ChIP & WB.
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect Dnmt1 in IP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19):8355-8365.).

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate Dnmt1 in ChIP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19):8355-8365.).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

质量

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 0.02 ?g/mL of this antibody detected Dnmt1 in 10 ?g of HeLa nuclear extract.

目标描述

~183 kDa observed. Three isoforms are known to exist at ~183 kDa, ~185 kDa, and ~145 kDa An uncharacterized band may be observed at ~90 kDa in some nuclear extracts.

外形

Format: Purified
Purified rat monoclonal IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa nuclear extract

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	rat
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	DNM-2C1, monoclonal
species reactivity	human
technique(s)	ChIP: suitable immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG2b
NCBI登记号	NP_001124295
UniProt登记号	P26358
运输	wet ice
Gene Information	human ... DNMT1(1786)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable