Anti-DNA polymerase delta p125 Antibody, clone 11E10.1

半岛bd体育手机客户端 说明

一般描述

DNA polymerase delta catalytic subunit (EC 2.7.7.7; UniProt P28340; also known as CDC2 homolog, DNA polymerase subunit delta p125) is encoded by the POLD1 (also known as CDC2, MDPL, POLD, CRCS10) gene (Gene ID 5424) in human. DNA replication in eukaryotes involves multiple enzymes including the B family polymerases Pol α, Pol δ, and Pol ?. While Pol α initiates DNA synthesis, Pol δ and Pol ? replace Pol α after primer extension and perform the bulk of DNA replication. Pol δ and Pol ? are heterotetramers in higher eukaryotes comprised of POLD1 and POLE as the respective catalytic subunit, as well as accessory subunits (POLD2/3/4 and POLE2/3/4) that interact with cofactors such as Proliferating Cell Nuclear Antigen (PCNA). Both POLD1 and POLE contain an exonuclease domain (a.a. 268–471 of POLE and 304–517 of POLD1) that proof-reading capability during DNA replication. In addition to DNA synthesis, Pol ? and Pol δ are believed to participate in chromosomal DNA repairs, including nucleotide excision repair (NER), ismatch repair (MMR) and repair of double strand breaks (DSBR). Germline exonuclease domain mutations (EDMs) in POLD1 and POLE cause polymerase proofreading-associated polyposis (PPAP). Tumors with EDMs exhibits dramatically increased base substitutions.

免疫原

GST-tagged recombinant protein corresponding to the N-terminus of human DNA polymerase delta p125.

应用

Western Blotting Analysis: 0.5 ?g/mL from a representative lot detected DNA polymerase delta p125 in 10 ?g of Ramos cell lysate.
Anti-DNA polymerase delta p125 Antibody, clone 11E10.1 is an antibody against DNA polymerase delta p125 Antibody for use in Western Blotting.

质量

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected DNA polymerase delta p125 in 10 ?g of Raji cell lysate.

目标描述

~125 kDa observed.

外形

Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	11E10.1, monoclonal
species reactivity	human
technique(s)	western blot: suitable
同位素/亚型	IgG2bκ
NCBI登记号	NP_001243778
UniProt登记号	P28340
运输	wet ice
Gene Information	human ... POLD1(5424)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable