半岛bd体育手机客户端 说明
一般描述
Divalent metal ion transporter 1 (DMT1) is a proton-coupled Fe2+ transporter that is essential for iron uptake in enterocytes and for transferrin-associated endosomal iron transport in many other cell types. DMT1 dysfunction is associated with several diseases such as iron overload disorders and neurodegenerative diseases. DMT1 can transport manganese, cobalt, cadmium, nickel, and vanadium. DMT1 is also involved in iron transport from acidified endosomes into the cytoplasm in erythroid precursor cells as well as liver internal metabolism of iron. Interestingly too, in mammalian cells, mitochondria receive most incoming iron, and the outer mitochondrial membrane appears to be a major location for DMT1, which appears to be the major importer of iron into the mitochondria.
免疫原
Recombinant protein corresponding to human DMT1.
应用
Western Blot Analysis: 2 ?g/ml of this antibody detected DMT1 in 50 ?g of mouse liver, spleen and duodenum tissue lysate (Schumann, K.,et al. (2010). Journal of Trace Elements in Medicine and Biology 24 (2010) 58–66)
Immunocytochemistry Analysis: A 1:100 dilution of this antibody from a representative lot detected DMT1 in Hela, A431, and N1H-3T3 cells
Research Sub Category
Ion & Transport Channels
Ion Channels & Transporters
This Anti-DMT1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of DMT1.
Research Category
Signaling
Metabolism
质量
Evaluated by Western Blotting in human placenta tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DMT1 in 10 ?g of human placenta tissue lysate.
目标描述
~ 62 kDa observed
外形
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | serum |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | human, mouse |
technique(s) | immunocytochemistry: suitable western blot: suitable |
NCBI登记号 | NP_000608 |
UniProt登记号 | P49281 |
运输 | dry ice |
Gene Information | human ... SLC11A2(4891) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
Sigma-Aldrich