Anti-Desmoglein-3, clone 5G11 Antibody

半岛bd体育手机客户端 说明

一般描述

Desmoglein-3 (DSG3) is a calcium binding membrane protein that is localized desmosome cellular junctions and interacts with plaque proteins and intermediate filaments at cell-cell adhesion points. Desmosomes are cell-cell junctions between epithelial, myocardial and other cells types. Strong intercellular adhesion is critical for tissues that experience mechanical stress, such as the skin and heart. Desmosomes provide adhesive strength to tissues by anchoring desmosomal cadherins of neighboring cells to the intermediate filament cytoskeleton. It is thought that raft membrane microdomains play a role in desmosome homeostasis by regulating the dynamics of desmosome assembly and disassembly. In human keratinocytes, Desmoglein-3 (DSG3) is raft associated and disruption of rafts prevents desmosome assembly. Desmoglein-3 is one of four sister proteins in the desmoglein family. Desmoglein-3 is also the autoantigen for Pemphigus vulgaris (PV) a lethal skin disease that is a result of autoantibodies against Desmoglein-3.

免疫原

Recombinant protein corresponding to human Desmoglein-3.

应用

Research Category
Cell Structure
Immunohistochemistry Analysis: A representative lot detected Isocitrate Desmoglein-3 in human scalp hair follicles and epidermal inclusions (Wu, H., et al. (2003). J Invest Dermatology. 120(6):1152-1157).
Immunohistochemistry Analysis: A representative lot detected Isocitrate Desmoglein-3 in in human adult skin tissue (Wu, H., et al. (2013). New England Journal of Med. 343(1):31-35).
This Anti-Desmoglein-3, clone 5G11 Antibody is validated for use in Immunohistochemistry (Paraffin) for the detection of Desmoglein-3.
Research Sub Category
Adhesion (CAMs)

质量

Evaluated by Immunohistochemistry in human tonsil and human skin tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Desmoglein-3 in human tonsil and human skin tissue.

目标描述

107 kDa calculated

联系

Replaces: MABT470

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	300
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	5G11, monoclonal
species reactivity	human
technique(s)	immunohistochemistry: suitable (paraffin)
同位素/亚型	IgG1κ
NCBI登记号	NP_001935
UniProt登记号	P32926
运输	wet ice
Gene Information	human ... DSG3(1830)

安全信息

储存分类代码

10 - Combustible liquids