Anti-DDR2 Antibody, clone 2B12.1

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一般描述

Discoidin domain-containing receptor 2 (EC 2.7.10.1; UniProt Q16832; also known as CD167 antigen-like family member B, CD167b, Discoidin domain receptor 2, Discoidin domain-containing receptor tyrosine kinase 2, Neurotrophic tyrosine kinase, receptor-related 3, Receptor protein-tyrosine kinase TKT, Tyrosine-protein kinase TYRO10) is encoded by the DDR2 (also known as NTRKR3, TKT, TYRO10) gene (Gene ID 4921) in human. Discoidin domain-containing receptors (DDRs) belong to the superfamily of transmembrane receptor tyrosine kinases (RTKs). DDRs are distinguished from other RTKs by their extracellular discoidin motif and their utilization of collagens as internal ligands. While DDR1 binds essentially all types of collagens, DDR2 shows a preference for type I, II and III fibrillar collagens, and nonfibrillar type X collagen. DDR1 is widely expressed in epithelial cells in lung, kidney, colon, and brain, whereas DDR2 is primarily expressed in mesenchymal cells including fibroblasts, myofibroblasts, smooth muscle cells, and chondrocytes in kidney, skin, lung, heart, and connective tissues. DDRs are important mediators of fundamental cellular processes, such as proliferation, survival, differentiation, adhesion and matrix remodeling, their dysregulations are reported to cause a number of human diseases, including fibrotic disorders, atherosclerosis and cancer. DDR2 is initially produced with a signal peptide sequence (a.a. 1-21), the removal of which yields the mature protein with an extracellular region (a.a. 22-399), followed by a transmembrane segment (a.a. 400-421) and a cytoplasmic region (a.a. 422-855) that harbors the kinase domain (a.a. 563-849).

免疫原

GST-tagged recombinant human DDR2 internal fragment.

应用

This Anti-DDR2 Antibody, clone 2B12.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of DDR2.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected DDR2 in 10 µg of rat L6 myoblast and human HeLa cell lysates.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected DDR2 in human lung and kidney tissue sections.

质量

Evaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DDR2 in 10 µg of NIH3T3 cell lysate.

目标描述

~107/115 kDa observed. Target band(s) appears larger than the calculated molecular weight of 96.74 kDa due to glycosylation. A doublet banding pattern is often seen with the lower band representing less glycosylated DDR2 species.

外形

Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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