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位置: 首页> 品牌> Sigma-Aldrich> Anti-DARPP-32 Antibody, clone 9B6.1

Anti-DARPP-32 Antibody, clone 9B6.1

品牌
Sigma-Aldrich
货号
MABN869
规格纯度
clone 9B6.1, from mouse
价格
5520.38 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-DARPP-32 Antibody, clone 9B6.1
Protein phosphatase 1 regulatory subunit 1B, DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Protein phosphatase 1 regulatory subunit 1B (EC 3.1.3.16; UniProt Q9UD71; also known as DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein 32) is encoded by the PPP1R1B (also known as DARPP32) gene (Gene ID 84152) in human. DARPP-32 is a PKA substrate that, upon phosphorylation on Thr34 by PKA, functions as a protein-phosphatase 1 (PP1) inhibitor. DARPP-32 N-terminal a.a. 8-11 (KIQF) is essential for PPI binding. DARPP-32 is an important mediator of dopamine receptor-dependent NMDA receptor activation. Dopamine receptor activation leads to cAMP induction, which activates cellular PKA activity and subsequent DARPP-32 Thr34 phosphorylation. PP1 inhibition by the Thr34 phosphorylated DARPP-32 in turn results in an enhnaced NMDA receptor phosphorylation. Calcium/calmodulin-dependent protein phosphatase 2B (PP2B or calcineurin) can reverse DARPP-32 Thr34 phosphorylation and negatively regulate its PP1 inhibitory activity. DARPP-32 is also known to be phosphorylated on Ser137 by casein kinase I (CKI), on Ser102 by CKII, and on Thr75 by Cdk5. Ser137 phosphorylation prevents PP2B from dephosphorylating pSer34, and Ser102 phosphorylation promotes Thr34 phosphorylation by PKA. Thr75 phosphorylated DARPP-32 is reported to exhibit PKA inhibitory activity in vitro.

特异性

Expected to react with both spliced isoforms of human DARPP-32 (UniProt Q9UD71-1 & Q9UD71-2).

免疫原

Epitope: C-terminal half.
GST-tagged recombinant protein corresponding to the C-terminal half of human DARPP-32.

应用

Western Blotting Analysis: 1.0 ?g/mL from a representative lot detected DARPP-32 in 10 ?g of rat brain tissue lysate.
Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected DARPP-32 in human cerebral cortex and human testis tissue.
Research Sub Category
Signaling Neuroscience
Research Category
Neuroscience
This Anti-DARPP-32 Antibody, clone 9B6.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of DARPP-32.

质量

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.0 ?g/mL of this antibody detected DARPP-32 in 10 ?g of human brain tissue lysate.

目标描述

~32/30 kDa observed. Target band(s) appears larger than the caluclated molecular weights of 22.96 kDa (isoform 1) and 18.74 kDa (Isoform 2).

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 9B6.1, monoclonal
species reactivity rat, human
technique(s) immunohistochemistry: suitable
western blot: suitable
同位素/亚型 IgG1κ
NCBI登记号 NP_001229393
UniProt登记号 Q9UD71
运输 wet ice
Gene Information human ... PPP1R1B(84152)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

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