Anti-Cytomegalovirus Antibody, immediate early, clone 7D12.2

半岛bd体育手机客户端 说明

特异性

Reacts with an immediate early protein M.W. 68 kD. Can detect CMV infection 1 hour post-infection exhibiting a nuclear and cytoplasmic staining which reaches peak intensity between 8-24 hours.

With CMV the antigens expressed at different times are listed as:



Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.

Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.

Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD

免疫原

MRC-5 cells infected with CMV AD169.
Epitope: immediate early

应用

Research Category
Infectious Diseases
This Anti-Cytomegalovirus Antibody, immediate early, clone 7D12.2 is validated for use in IF, WB, IC, IH for the detection of Cytomegalovirus.
Immunohistochemistry.

Immunocytochemistry.

IFA at 1:3,000-1:6,000 on acetone fixed cells.

Does not work on paraffin embedded tissue sections.

Dilute with buffer pH 7.4-7.6 to desired working volume.

For extensive dilution, protein containing or other stabilizing medium should be used.

Final working dilutions must be determined by end user.
Research Sub Category
Infectious Diseases - Viral

外形

Purified ascites in 0.02 M Phosphate Buffered Saline, 0.25 M Sodium Chloride, 0.1% Sodium Azide,

pH 7.6 .
Format: Purified

储存及稳定性

Maintain at +2° to + 8°C for up to 12 months from date of receipt.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160702

半岛bd体育手机客户端 性质

质量水平	300
生物来源	mouse
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	7D12.2, monoclonal
species reactivity	human
manufacturer/tradename	Chemicon?
technique(s)	immunocytochemistry: suitable immunofluorescence: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG1
适用性	not suitable for immunohistochemistry (Paraffin)
运输	wet ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable