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位置: 首页> 品牌> Sigma-Aldrich> Anti-Cytokeratin 5,6 Antibody, clone D5/16B4, Alexa Fluor?555
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Anti-Cytokeratin 5,6 Antibody, clone D5/16B4, Alexa Fluor?555

品牌
Sigma-Aldrich
货号
MAB1620-AF555
规格纯度
clone D5/16B4, from mouse, ALEXA FLUOR?555
价格
6526.19 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
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半岛bd体育手机客户端 名称:
Anti-Cytokeratin 5,6 Antibody, clone D5/16B4, Alexa Fluor ?555
Keratin, type II cytoskeletal 5, 58 kDa cytokeratin, CK-5, Cytokeratin-5, K5, Keratin-5, Type-II keratin Kb5
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Keratin, type II cytoskeletal 5 (UniProt P13647; also known as 58 kDa cytokeratin, CK-5, Cytokeratin-5, K5, Keratin-5, Type-II keratin Kb5) is encoded by the KRT5 (also known as DDD1, DM-EBS, EBSMCE, K-EBS, MP-EBS, WC-EBS) gene (Gene ID 3852) in human. Cytokeratin-5 (Keratin-5 or K5) belongs to a family of highly conserved epithelial-specific intermediate filament (IF) proteins. Each epithelium is characterized by its unique keratin expression pattern. In interfollicular epidermis, four major keratins are synthesized and co-polymerize in pairs, K5/K14 and K1/KI0, to form filaments. The expression of these two pairs is strictly regulated during normal keratinocyte differentiation. The K5/K14 filament is expressed in proliferating basal cells, while the K1/K10 filament is expressed during the transition to terminal differentiation and at the cessation of cell division. Similar to other IF members, Keratin-5 contains a central rod region composed of alpha helix segments (a.a. 168-203, 223-315, 339-477) that allow two different IF proteins, one type I (acidic) and one type II (basic) keratin, to intertwine into a coiled-coil formation via hydrophobic interaction. Kearain-5 is a type II keratin that pairs with the type I keratin-14 to form IF.

特异性

Clone D5/16B4 detected cytokeratin 5 by Western blotting using cytoskeletal preparations from human epidermis and non-cytokeratinizing epithelium (Abstract: European Symposium of the Biology of the Cytoskeleton, Helsinki, 18-21, 6, 89, 1989), as well as by staining tissue sections. Clone D5/16B4 also shows reactivity toward cytokeratin 6 and weak reactivity with cytokeratin 4 by Western blotting. No cross-reactivity to cytokeratin 1, 7, 8, 10, 13, 14, 18 or 19. Clone D5/16B4 immunostains basal cells and a part of the stratum spinosum in the normal pavement epithelium. Clone D5/16B4 is suitable for staining formalin-fixed tissue sections.

免疫原

Purified human cytokeratin 5 (Mischke, D., and Wild, G.A. (1987). J. Invest. Dermatol. 88(2):191-197; Wild, G.A., and Mischke, D. (1986). Exp. Cell Res. 162(1):114-126).

应用

Research Category
Cell Structure
The unconjugated antibody (Cat. No. MAB1620) is shown to be suitable also for immunofluorescence, immunohistochemistry, and Western blotting applications.
Anti-Cytokeratin 5,6 Antibody, clone D5/16B4, Alexa Fluor?555 is an antibody against Cytokeratin 5,6 for use in Immunocytochemistry.
Research Sub Category
Cytoskeleton

质量

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Cytokeratin 5/6 in A431 cells.

目标描述

Cytokeratin 4 (59 kDa), Cytokeratin 5 (58 kDa) and Cytokeratin 6 (56 kDa).

外形

Purified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Protein A purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

法律信息

ALEXA FLUOR is a registered trademark of Life Technologies

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
偶联物 ALEXA FLUOR?555
抗体形式 purified antibody
antibody product type primary antibodies
克隆 D5/16B4, monoclonal
species reactivity mouse, rat, human
technique(s) immunocytochemistry: suitable
同位素/亚型 IgG1
NCBI登记号 NM_000424.3
UniProt登记号 P13647
运输 wet ice
Gene Information human ... KRT5(3852)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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