Anti-Cytochrome P450 Aldosterone Synthase Antibody, clone 1G5

半岛bd体育手机客户端 说明

一般描述

Aldosterone synthase (or 18-hydroxylase) is a steroid hydroxylase cytochrome P450 oxidase enzyme involved in the generation of aldosterone. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency. (Pascoe, L, et al. (1992) "Mutations in the human CYP11B2 (aldosterone synthase) gene causing corticosterone methyloxidase II deficiency." PNAS, USA. 89:496-5000).

特异性

Rat cytochrome p450 aldosterone synthase

免疫原

Aldo-1-MAP (aa 175-192) (KVRQNARGSLTMDVQQSL-MAP)

应用

Western blot: recognizes ~48kDa band in adrenal zona glomerulosa mitochondrial preparations. The enzyme is expressed best in adrenal zona glomerulosa tissues, expression in kidney is virtually undetectable.
Immunohistochemistry: 1:50-1:200 , ABC, enzymatic detection; clone works best with STF fixed tissues that are paraffin embedded. STF is available from Streck Laboratories, www.streck.com. Formalin fixation has been successfully reported but working dilutions are much lower (see Fujisawa, et al). Recommended counter stain is Gill′s Haematoxylin.
Immunocytochemistry: acetone fixed cells, 1:20-1:200.
ELISA
Optimal working dilutions must be determined by end user.

This Anti-Cytochrome P450 Aldosterone Synthase Antibody, clone 1G5 is validated for use in ELISA, IC, IH(P), WB for the detection of Cytochrome P450 Aldosterone Synthase.

外形

Unpurified tissue culture supernatant containing 50% glycerol and 0.1% sodium azide as a preservative.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	culture supernatant
antibody product type	primary antibodies
克隆	1G5, monoclonal
species reactivity	rat, mouse
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable immunocytochemistry: suitable immunohistochemistry: suitable (paraffin) western blot: suitable
同位素/亚型	IgG1κ
UniProt登记号	P30099
运输	wet ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1