Anti-Cytochrome P450 11β-Hydroxylase Antibody, clone 11B2-1A4

半岛bd体育手机客户端 说明

特异性

Rat p450 11beta-Hydroxylase

免疫原

Aa 272-285 (KNVYRELAE GRQQS-C)

应用

Anti-Cytochrome P450 11β-Hydroxylase Antibody, clone 11B2-1A4 is an antibody against Cytochrome P450 11β-Hydroxylase for use in IH(P) & WB.
Western blot: 1:300

Immunohistochemistry (S.T.F. on paraffin embedded tissue required, traditional formalin fixation or paraformaldehyde fixation is not recommended. Microwave or pressure cooker antigen retrieval; blocking with a solution of 0.5% SDS, 2% milk and 5% NGS in Tris-HCL pH 7.6. Primary antibody is diluted in PBS or TBS typically with no SDS or NGS: 1:50-200, usually overnight refrigerated. After washing 4X with Tris buffer pH 7.6, secondary goat anti-mouse secondary (AP124B) is incubated (1:500-1:2000) diluted in a solution of 2% milk, 5% NGS, Tris-HCL pH 7.6 and 0.05% tween 20. Detection is via streptavidin HRP/DAB. Recommended counter stain is Gill′s Haematoxylin.

Optimal working dilutions must be determined by end user.

STF fixative is available from streck laboratories, www.streck.com).
Research Category
Metabolism
Research Sub Category
Enzymes & Biochemistry

外形

Supernatant. Liquid in 0.1% sodium azide.

储存及稳定性

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	culture supernatant
antibody product type	primary antibodies
克隆	11B2-1A4, monoclonal
species reactivity	rat
manufacturer/tradename	Chemicon?
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
同位素/亚型	IgG1
UniProt登记号	P30099
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable