Anti-Cullin-3 Antibody, clone 3A8.1

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一般描述

Cullin-3 (UniProt Q13618) is encoded by the CUL3 (also known as PHA2E, KIAA0617) gene (Gene ID 8452) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. Cullin-3 belongs to the cullin family of hydrophobic proteins that confer substrate specificity to the multimeric Cullin-RING E3 ubiquitin ligase (CRL) complexes by acting as scaffold proteins. The activity of CRLs is regulated by Cullin Neddylation via the reversible conjugation of a small ubiquitin-type protein known as Neural precursor cell expressed developmentally down-regulated protein 8 (Nedd8). Cullin-3-based CRLs rely on Cullin-3 interaction with bric-a-brac/tramtrack/broad-complex (BTB) domain-containing proteins for targets recruitment.

免疫原

Epitope: C-terminal
GST-tagged recombinant protein corresponding to the C-terminal of human Cullin-3.

应用

Research Sub Category
Apoptosis - Additional
This Anti-Cullin-3 Antibody, clone 3A8.1 is validated for use in Western Blotting for the detection of Cullin-3.
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in mouse neuroblastoma Neuro-2a (N2a) cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected Cullin-3 in 10 ?g of mouse neuroblastoma Neuro-2a (N2a) cell lysate.

目标描述

~88 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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