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位置: 首页> 品牌> Sigma-Aldrich> Anti-CRISPR/CAS9 -FITC antibody, Mouse monoclonal

Anti-CRISPR/CAS9 -FITC antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
SAB4200735
规格纯度
clone 7A9-3A3, purified from hybridoma cell culture
价格
6401.96 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-CRISPR/CAS9 -FITC antibody, Mouse monoclonal
Anti-Crispr RNA
Anti-Crispr
Anti-CRISPR-associated protein-9 nuclease
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) belongs to the type II CRISPR/CAS9 system. It is part of an adaptive immune system of the Streptococcus pyogenes SF370, protecting from pathogens′ target genes by cleaving the foreign DNA in a sequence-dependent manner.
The type II CRISPR/Cas system has been adapted to expression in eukaryotic cells. The Cas9 endonuclease can be engineered with a single gRNA (guide RNA), directing a DNA double-strand break (DSB) at a desired genomic location. As a result, the cell activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR). In comparison to other genome-editing technologies such as designer zinc fingers (ZFs), transcription activator–like effectors (TALEs) and homing meganucleases, the CRISPR/CAS9 system is a scalable, affordable and easy to engineer. Therefore, the anti-CRISPR/CAS9-FITC conjugated antibody can be a useful tool for detecting CRISPR/CAS9 positively transfected cells, revealing DSB sites in their genome.

免疫原

Recombinant protein within the N-terminal region of Streptococcus pyogene Cas9

应用

Monoclonal Anti-CRISPR/CAS9-FITC recognizes the CAS9 protein in CAS9 construct over-expression systems. The antibody may be used in various immunochemical techniques including Immunofluorescence. The antibody successfully recognizes mutant Cas9 variants, including nickase Cas9 and dead Cas9 (dCas9) and does not cross react with FnCas9 from Francisella novicida bacteria and Cpf1 proteins from Acidaminococcus sp. (strain BV3L6) and Lachnospiraceae bacterium ND2006.

外形

Solution in 0.01M phosphate buffered saline pH 7.4 containing 1% bovine serum albumin and 15mM sodium azide.

储存及稳定性

For continuous use, store at 2-8℃, protected from light for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

其他说明

This product is for R&D use only, not for drug, household, or other uses.
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

半岛bd体育手机客户端 性质

质量水平 200
偶联物 FITC conjugate
抗体形式 purified from hybridoma cell culture
antibody product type primary antibodies
克隆 7A9-3A3, monoclonal
形式 buffered aqueous solution
储存条件 protect from light
浓度 ~1.0 mg/mL
technique(s) immunofluorescence: 1:200-1:400 using human HEK-293T cells over-expressing CAS9 protein
同位素/亚型 IgG1
UniProt登记号 Q99ZW2
运输 dry ice
储存温度 ?20℃

安全信息

储存分类代码 12 - Non Combustible Liquids
闪点(F) Not applicable
闪点(C) Not applicable

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