Anti-Collagen Type II Antibody, clone 6B3

半岛bd体育手机客户端 说明

特异性

In Western blotting (but not ELISA), MAB8887 recognizes both a1(II) and a3(XI) chains which have identical primary structure. In Western blotting, MAB8887 reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. It also reacts with pepsin-digested type II collagen. It will not react with intact Collagen II under traditional conditions.

Its epitope is localized in the triple helix of type II collagen. It shows no cross-reaction with type I or type III collagen. Immunoblotting of CNBr peptides of collagen II shows that MAB8887 reacts with CB11 (25kDa) fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule.

In pepsin solublized collagen II, MAB8887 reacts with a 95-97 kDa fragment, as well as, the native single chain of 120kDa. If propeptides are present, MAB8887 will detect a 200kDa fragment.

免疫原

Purified type II collagen; obtained from adult chicken sternum by limited pepsinization.

应用

Research Category
Cell Structure
Anti-Collagen Type II Antibody, clone 6B3 is an antibody against Collagen Type II for use in IF, IH & WB.
Western Blot (Mayne, 1994)

Immunohistochemistry (Formalin/Paraffin): 1-2 μg/mL, 30 minutes at room temperature. Staining of paraffin embedded tissues requires digestion of tissue sections with pepsin at 1mg/mL in Tris HCl, pH 2.0 for 15 minutes at RT or 10 minutes at 37?C.

Immunofluorescence

ELISA

Optimal working dilutions must be determined by end user.
Research Sub Category
ECM Proteins

目标描述

120 kDa

外形

Purified immunoglobulin in 10 mM PBS, pH 7.4 with 0.2% BSA and 0.09% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Maintain at 2–8°C in undiluted aliquots for up to 12 months

分析说明

Control
Cartilage in lung or fetus

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	6B3, monoclonal
species reactivity	chicken, human, salamander, mouse
manufacturer/tradename	Chemicon?
technique(s)	immunofluorescence: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG1
NCBI登记号	NM_001844.4 NM_033150.2
UniProt登记号	P02458
运输	wet ice
Gene Information	human ... COL2A1(1280)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable