Anti-Chondroitin Sulfate Proteoglycan Antibody, clone 9.2.27

半岛bd体育手机客户端 说明

特异性

Recognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250 kDa as well as precursor polypeptides of 210, 220 and 240 kDa (Bumoi et al., 1984). Reacts with human melanoma, glioma and proliferating brain endothelial cells.

免疫原

Human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose (Morgan et al., 1981).

应用

Research Category
Cell Structure
Detect Chondroitin Sulfate Proteoglycan using this Anti-Chondroitin Sulfate Proteoglycan Antibody, clone 9.2.27 validated for use in FC, IF & IP.
Immunofluorescence microscopy of cells in culture (Bumoi et al., 1984; Schrappe et al., 1992). Also, Legg J et al (2003) Development 130:6049-6063 {3% PFA fixed frozen sections}.

Immunoelectron microscopy (Bumoi et al., 1984)

Immunoprecipitation: 1μg/10E6 cells (Bumoi et al., 1984; Morgan et al., 1981)

FACS analysis: 1-2μg/10E6 cells

Functional block of melanoma cell spreading (Morgan et al., 1981), glioma cell proliferation3 and tumor growth in nude mice (Morgan et al., 1981; Schrappe et al., 1992).

Optimal working dilutions must be determined by end user.
Research Sub Category
ECM Proteins

外形

Purified immunoglobulin in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Format: Purified

储存及稳定性

Maintain between 2 and 8°C in undiluted aliquots for up to 6 months.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	9.2.27, monoclonal
species reactivity	human
manufacturer/tradename	Chemicon?
technique(s)	flow cytometry: suitable immunofluorescence: suitable immunoprecipitation (IP): suitable
同位素/亚型	IgG2a
NCBI登记号	NM_001897.4
UniProt登记号	Q6UVK1
运输	wet ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable