Anti-Chondroitin 4 Sulfate Antibody, clone BE-123

半岛bd体育手机客户端 说明

特异性

Reacts with both mouse (fetal and adult) and human (fetal and adult) chondroitinase ABC digested cartilage proteoglycans. Chondroitin-4 sulfate disaccharides successfully inhibit the binding of monoclonal antibody to proteoglycans. No reaction with native proteoglycans or with proteoglycans digested with either chondroitinase AC or testicular hyaluronidase.

免疫原

Mouse proteoglycan.

应用

Research Category
Cell Structure
Anti-Chondroitin 4 Sulfate Antibody, clone BE-123 detects level of Chondroitin 4 Sulfate & has been published & validated for use in ELISA, RIA & WB.
ELISA at 1:400-1:3,200.

RIA at 1:50-1:6,400.

Immunohistochemistry: This antibody only reacts with digested material. Fixed tissue must be digested with chondroitinase ABC (Sigma).
The Reference 23757342 should be indicated for IHC. (Reine, TM., et al., PubMed ID: 23757342)

Western Blot: 1:1000-1:4000 using ECL detection method. Chondroitinase ABC treatment of lysate sample is helpful.
In any alkali buffer, e.g. Tris or acetate (50mM or 100mM) pH 7.4-7.6. Make a stock solution by diluting one vial of Sigma Chondroitinase A, B, C, (5 units/vial) in 100 ?L . For use, dilute stock 10-fold into the buffer of choice. Use at a ratio of 50-100mg of proteoglycan per 5 units of enzyme. On fixed cells, dilute in Tris acetate and on non-fixed cellsy dilute 10-25 fold in DMEM.
Poole, C.A., et al., J. Histochem. Cytochem. 1991. 39:1175-1187.

Optimal working dilutions must be determined by the end user.
Research Sub Category
ECM Proteins

外形

Ascites fluid containing no preservatives.

储存及稳定性

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	BE-123, monoclonal
species reactivity	mouse, sheep, bovine, human, canine, rat
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable radioimmunoassay: suitable western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NM_001897.4
UniProt登记号	Q6UVK1
运输	dry ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable