半岛bd体育手机客户端 说明
一般描述
Cholera toxin (CTx) also known as choleragen, is an enterotoxin produced by the Gram-negative bacterium Vibrio cholerae that naturally habitat in fresh or saltwater environments. Most of theV. choleraespecies do not cause any disease in human, but few including serotypes O1 and O139 can cause cholera pandemic. These cases were described already in the 19th century.1 TheV. choleraevirulence factors CtxA and CtxB are located at the CTX phage genome integrated within the bacterial chromosome. Since species virulence may change due to mutations and acquisition of virulence genes, the cholera pandemic has a major public health risk with potential for large numbers of cases and even deaths.1-3
特异性
Monoclonal Anti-Cholera Toxin-peroxidase antibody specifically recognizes Cholera Toxin and has no cross reactivity withStaphylococcal EnterotoxinA (SEA),Staphylococcal EnterotoxinB (SEB),Pseudomonas Exotoxin AorStaphylococcal Alpha-Toxin(α-Hemolysin).
免疫原
recombinant Cholera toxin beta (C-terminal His-tag) protein.
应用
The antibody may be used in various immunochemical techniques including Immunoblotting, Immunofluorescence and ELISA. Detection of the CTxB band by Immunoblotting is specifically inhibited by the immunogen.
生化/生理作用
CTx is composed of two subunits, the toxic CTxA (~27 kDa) and non-toxic CTxB (~12 kDa) assembled with the stoichiometry AB5.4 The B-subunit specifically binds to monosialogangliosides GM1 receptors, located in the membrane of intestinal epithelial cells.5 The A1 fragment of the A-subunit is translocated through the membrane of the host cell, where it catalyses the ADP-ribosylation of the Gsa regulatory component of the adenylate cyclase complex. The resulting increased level of cyclic AMP promotes a wide variety of actions, including the secretion of chloride ions in the case of intestinal epithelial cells.6-7 Antibodies specific for cholera toxin may be used in studies of structural and functional aspects of toxin-membrane interactions and for the detection of CTxB when used for example as an adjuvant when injected mucosally together with the desired antigen.8-10
外形
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
储存及稳定性
For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses,in vitrodiagnostic uses,ex vivoorin vivotherapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | CTxB-24, monoclonal |
| 描述 | Research area: Microbiome |
| species reactivity | Vibrio cholerae |
| 包装 | antibody small pack of 25 μL |
| 浓度 | ~1 mg/mL |
| technique(s) | immunoblotting: 1-2 μg/mL using His-tagged recombinant Cholera Toxin B subunit |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P01556 |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
