Anti-CD42a Antibody, platelet lb-IX, clone FMC-25

半岛bd体育手机客户端 说明

特异性

This antibody belongs to CD42a (assigned at the Fourth International Workshop on Leukocyte Differentiation Antigens, Vienna, 1989) (Knapp, 1989), and binds to the 22,000 dalton IX subunit of the glycoprotein Ib-IX complex (Zola et al., 1984; Berndt et al., 1985a,b). FMC-25 failed to bind to platelets from patients with Bernard-Soulier Syndrome (Berndt et al., 1983). The antibody is specific for cells of the platelet megakaryocyte lineage. It reacts with a proportion of acute myeloid leukemias being useful in identifying those of the megakaryoblastic subtype (San Miguel et al., 1985, 1986).

Cell reactivity:

The antibody is specific for cells of the platelet megakaryocyte lineage. It reacts with a proportion of acute myeloid leukemias being useful in identifying those of the megakaryoblastic subtype(San Miguel et al., 1985, 1986).

免疫原

Peripheral blood mononuclear cells isolated on Ficoll^?hypaque.
Epitope: platelet lbalpha complex

应用

Research Sub Category
Immunoglobulins & Immunology
Anti-CD42a Antibody, platelet lb-IX, clone FMC-25 detects level of CD42a & has been published & validated for use in FC, IC, IH.
Research Category
Inflammation & Immunology
The glycoprotein Ib-IX complex on the surface of normal platelets is a heterotrimer of Ib-alpha, Ib-beta and IX subunits (Berndt, 1989). Ib-IX complex is an essential component of platelet adhesion to vascular subendothelium and is also important in the maintenance of platelet shape (Berndt et al., 1988).

This antibody is suitable for enumeration of platelets by flow cytometry and immunoperoxidase staining of frozen tissue sections or air dried smear preparations (San Miguel et al., 1986), and for identification of megakaryoblasts in acute myeloid leukemia or acute myelofibrosis (San Miguel et al., 1985; San Miguel et al., 1986).

SUGGESTED USAGE DILUTION

1. Flow cytometry and indirect immunofluorescence 1:25

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 platelets in 100 μl of buffer.

2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:20 will be applicable to most commonly used systems.

外形

Purified immunoglobulin. The antibody is supplied in 25mM Tris-HCl, 0.4M NaCl, pH 8.0 containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.
Format: Purified

储存及稳定性

Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Ficoll is a registered trademark of Cytiva

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	FMC-25, monoclonal
species reactivity	human
manufacturer/tradename	Chemicon?
technique(s)	flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry: suitable
同位素/亚型	IgG1
NCBI登记号	NM_000174.2
UniProt登记号	P14770
运输	wet ice

安全信息

象形图
警示用语：	Warning
危险声明	H290
预防措施声明	P234 - P390
危险分类	Met. Corr. 1
储存分类代码	8B - Non-combustible, corrosive hazardous materials
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable