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Anti-Calponin antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
SAB4200710
规格纯度
clone hCP, purified from hybridoma cell culture
价格
6162.13 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-Calponin antibody, Mouse monoclonal
Anti-CNN-2
Anti-CNN-1
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Anti-Calponin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hCP hybridoma (also cites as CALP), produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse. Calponin (CNN) exists in three isoforms CNN1, CNN2 and CNN3. CNN2 gene is mapped to human chromosome 19p13.3. All three isoforms are expressed in smooth muscle. While CNN1 expression is confined only to smooth muscle, CNN2 and CNN3 are expressed in other cells including the neuronal tissue, fibroblast, myoblast and B lymphocyte. The three isoforms differ in the C-terminal region and comprise conserved actin-binding middle domain and a N-terminal calponin homology (CH) domain.

特异性

Monoclonal Anti-Calponin specifically recognizes smooth muscle Calponin.

免疫原

human uterus smooth muscle extract

应用

Anti-Calponin antibody, Mouse monoclonal may be used in:

  • immunoblotting
  • immunoprecipitation
  • immunohistochemistry

生化/生理作用

Calponin is an F-actin, calmodulin, and tropomyosin binding protein that presents in both the cytoskeleton and contractile machinery of smooth muscle (SM) cells. The interaction of Calponin with actin is shown to inhibit myosin Mg-ATPase activity. The binding strength of calponin to tropomyosin is weakened in the presence of Ca2+. Calponin is involved in a variety of cellular processes including smooth muscle contraction, cell movement, shape change, angiogenesis, exocytosis and endocytosis. Monoclonal anti-Calponin can be used for the identification and localization of calponin in SM elements. Calponin levels may serve as a prognostic factor in hepatocellular carcinoma, renal cell carcinoma, osteosarcoma and melanoma.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

储存及稳定性

For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES NA.41

半岛bd体育手机客户端 性质

抗体形式 purified from hybridoma cell culture
antibody product type primary antibodies
克隆 hCP, monoclonal
形式 buffered aqueous solution
分子量 antigen ~34 kDa
species reactivity rabbit, pig, canine, hamster, mouse, monkey, bovine, rat, ferret, human
浓度 ~1 mg/mL
technique(s) immunoblotting: suitable
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human tonsil sections
immunoprecipitation (IP): suitable
同位素/亚型 IgG1
UniProt登记号 Q99439
运输 dry ice
储存温度 ?20℃

安全信息

储存分类代码 12 - Non Combustible Liquids
闪点(F) Not applicable
闪点(C) Not applicable

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