Anti-Calpain Antibody, small subunit of μ- or m-Calpains (Calpain I or II), clone P1

半岛bd体育手机客户端 说明

一般描述

Calpains are non-lysosomal cysteine proteeases which absolutely require calcium for activity. There are two major calpain isozymes: μ-calpain and m-calpain, which require micromolar or millimolar calicum for activity, respectively, in invitro assays. The major calpain isozymes are composed of two subunits: an 80kDa catalytic subunit; and a 30kDa regulatory subunit. The large and small subunits remain associated in the presence of calcium after proteolytic activation of the zymogen.

特异性

Reacts with the small subunit of human erthrocyte μ-calpain (Calpain I) or bovine myocardial m-calpain (Calpain II).

免疫原

Bovine myocardial m-calpain.
Epitope: small subunit of μ- or m-Calpains (Calpain I or II)

应用

This Anti-Calpain Antibody, small subunit of μ-or m-Calpains (Calpain I or II), clone P1 is validated for use in ELISA, WB, IC, IH for the detection of Calpain.
Immunoblot: 1:500-1,000. The small subunit of either μ-calpain m-calpain is 30kDa. ELISA: 1:50,000 (against the immunogen). Immunohistochemistry: 1:100 on 4% paraformaldehyde or 1% paraformaldehyde/4% glutaraldehyde fixed tissue. Immunocytochemistry: 1:100. Optimal working dilutions must be determined by the end user.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	P1, monoclonal
species reactivity	human, bovine
should not react with	rat
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG1
NCBI登记号	NM_001003962.1 NM_001749.2
UniProt登记号	P04632
运输	dry ice
Gene Information	human ... CAPNS1(826)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable