Anti-c-K-Ras Antibody, clone 234-4.2

半岛bd体育手机客户端 说明

一般描述

GTPase KRas (c-k-ras; K-ras) is a membrane-bound G protein belonging to the family of Ras GTPases. They are activated by the binding of GTP in response to signals from various receptors including the epidermal growth factor receptor (EGFR). Ras GTPases are regulated by guanine nucleotide exchange factors (GEF) and GTPase activating proteins (GAP) and lie upstream of the MAP kinase pathway which controls a range of cellular processes including proliferation and differentiation. Previous studies have suggested that KRas plays an important role in embryonic development, and it may also contribute to the pathogenesis of colorectal cancer via an EGFR-mediated pathway.

免疫原

Recombinant protein corresponding to rat c-K-Ras.

应用

This Anti-c-K-Ras Antibody, clone 234-4.2 is validated for use in Western Blotting, IHC for the detection of c-K-Ras.
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
Research Category
Signaling
Western Blot Analysis: A representative lot detected c-K-Ras in A431 cell lysate.

Western Blot Analysis: A representative lot from an independent laboratory detected c-K-Ras in primary human
lymphoid leukemia cell lysates, and in normal and HA-dynamin K44A mutant HeLa cell lysates (Omerovic, J., et al. (2008). Oncogene. 27(19):2754-2762.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected c-K-Ras in non-small cell lung cancer cells (Volm, M., et al. (2002). Clin Cancer Res. 8(6):1843-1848.).

质量

Evaluated by Western Blot in SW-480 cell lysate.

Western Blot Analysis: 1 ?g/mL of this antibody detected c-K-Ras in 10 ?g of SW-480 cell lysate.

目标描述

~22 kDa observed

外形

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
SW-480 cell lysate

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	234-4.2, monoclonal
species reactivity	human
species reactivity (predicted by homology)	rat (immunogen homology)
technique(s)	immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_113703
UniProt登记号	P08644
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable