Anti-Bub1 Antibody, clone 14H5

半岛bd体育手机客户端 说明

一般描述

The mitotic checkpoint prevents the resulting two daughter cells from having unequal numbers of chromosomes. This condition, known as aneuploidy, is present in almost all cancer cells and may be due to malfunctions in the mitotic checkpoint. Bub1 is a mitotic checkpoint kinase and has been implicated in the metaphase checkpoint control in mammalian cells.

特异性

Recognizes human Bub1.

免疫原

Recombinant human Bub1.

应用

Western blot using an ECL detection system. Reacts with the ~150 kDa Bub1 protein.

Immunocytochemistry: 1 μg/mL on tissue culture cells. Suggested fix is 3.5% PBS-buffered paraformaldehyde for 7 minutes. Permeabilization method is 0.2% Triton-X-100 after fixation. Suggested blocking buffer is 10 mM Tris, pH 7.5 with 150 mM NaCl with 0.1% BSA.

Immunoprecipitation: 1 μg/mL. Preferred cell lysis buffer is RIPA. Final reaction volume is 200 μL with a final protein concentration in the reaction mix of 1 mg/mL. Suggested capture agent is Protein G.

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Detect Bub1 with Anti-Bub1 Antibody, clone 14H5 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC.

外形

Mouse IgG1 in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide.
Format: Purified

储存及稳定性

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	14H5, monoclonal
species reactivity	primate, human
manufacturer/tradename	Chemicon?
technique(s)	immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG1
NCBI登记号	NM_004336.2
UniProt登记号	O43683
运输	wet ice
Gene Information	human ... BUB1(699)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable