您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> Anti-β-Tubulin IV antibody, Mouse monoclonal

Anti-β-Tubulin IV antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
SAB4200792
规格纯度
clone ONS.1A6, purified from hybridoma cell culture
价格
6333.77 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-β-Tubulin IV antibody, Mouse monoclonal
Anti-TUBB4
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Tubulin is the major building block of microtubules. It possesses an intracellular cylindrical filamentous structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, Tubulin exists in all cells as a heterodimer of two similar but not identical polypeptides (approx. 55 kDa each), designated α and β, that assembles into microtubules. Both α and β Tubulins undergo post-translational modifications, including acetylation, phosphorylation, detyrosination, polyglutamylation and polyglycylation. For β-tubulin, six evolutionarily conserved isotypes were identified, designated βI-βVI. Their utilization in the same cell type of different species is nearly absolutely conserved with the exception of the sequenced diversed hematopoietic β-tubulin. Since the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules, it is hypnotized that the β-tubulin isotypes contribute to unique functional properties. The most complex pattern of isotype distribution in tissues is seen in the vertebrate β-tubulins. In mammals βI is constitutive and found in most tissues. βII is found in many tissues, but largely in the brain; its synthesis increases in regeneration and development of neurons. βIII is found in the brain and in dorsal root ganglia; it appears to be localized to neurons, where its expression seems to increase during axonal outgrowth. βIV in mammals exists at two subtypes, differing from each other at 10 positions. βIVa is brain specific and βIVb is ubiquitous, but both appear to be constitutive. βV is ubiquitous expressed in a variety of cultured mammalian cells. βVI is apparently restricted to hematopoietic tissues, being expressed in mammalian platelets, spleen, bone marrow and other blood-forming tissues. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis, organellar and flagellar movement, intracellular transport and cytoskeletal functions. Antibodies reacting specifically with α- and β-tubulin isotypes serve as an essential tool in the detection of the presence and functional significance of these molecules in various cellular settings.

免疫原

Synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype IV, conjugated to BSA.

应用

Immunoblotting: a working concentration of 10-20 μg/mL is recommended using human breast cancer MCF7 cell line.
Immunofluorescence: a working concentration of 5-10 μg/mL is recommended using human foreskin Hs68 cells.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide.

其他说明

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

基本信息

NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
抗体形式 purified from hybridoma cell culture
antibody product type primary antibodies
克隆 ONS.1A6, monoclonal
形式 buffered aqueous solution
分子量 ~55 kDa
species reactivity rat, human, chicken, bovine, mouse
technique(s) ELISA: suitable
immunoblotting: 10-20 μg/mL using human breast cancer MCF7 cell line.
immunofluorescence: 5-10 μg/mL using human foreskin Hs68 cells.
immunohistochemistry: suitable
UniProt登记号 P04350
运输 dry ice
储存温度 ?20℃

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map