Anti-AS160 Antibody

半岛bd体育手机客户端 说明

一般描述

AS160 (Akt Substrate of 160 kDa, Akt1S1), Rab GAP (GTPase activating protein), is phosphorylated on multiple sites by Akt in response to insulin in adipocytes and contraction in muscles. AS160 is the long sought after link between the upstream insulin signaling through insulin/PI3K/Akt and GLUT4 vessicle translocation to the cell membrane. It has 6 Akt phosphorylation consensus sequences that are believed to become phosphorylated upon insulin stimulation. This phosphorylation is required for translocation of GLUT4-containing vesicles to the cell membrane1. A mutation of two or more of these phosphorylation sites results in a dramatic decrease in AS160 activity and insulin-stimulated GLUT4 redistribution at the cell membrane2. AS160 has also shown to be important for GLUT4 expcytosis2.

特异性

This antibody recognizes AS160 at the C-terminus.
Chimpanzee, equine, and ox (90% sequence homology).

免疫原

KLH-conjugated linear peptide corresponding to the C-terminus of mouse AS160.
Epitope: C-terminus

应用

Western Blot (SNAP ID) Analysis: 1 ?g/mL from a previous lot detected AS160 on 10 ?g of HEPG2 cell lysate.

Immunoprecipitation Analysis: 10 ?g from a previous lot immunoprecipitated AS160 from HEPG2 lysate.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
Anti-AS160 Antibody detects level of AS160 & has been published & validated for use in WB & IP.

质量

Evaluated by Western Blot in HEPG2 cell lysate.

Western Blot Analysis: 0.05 ?g/mL of this antibody detected AS160 on 10 ?g of HEPG2 cell lysate.

目标描述

~ 160 kDa

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HEPG2 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human, mouse
species reactivity (predicted by homology)	rat (based on 100% sequence homology), canine (based on 100% sequence homology)
technique(s)	immunoprecipitation (IP): suitable western blot: suitable
NCBI登记号	NP_001074747
UniProt登记号	Q8BYJ6
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable