Anti-APP-C99 Antibody, clone mC99(1-7)

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一般描述

Amyloid Precursor Protein (APP) is expressed in the brain, kidney, heart, and spleen of fetal tissues; it is induced during neuronal differentiation. The most-substantiated role for APP is in synaptic formation and repair; its expression is upregulated during neuronal differentiation and after neural injury. In adult brain, highest expression of APP is found in the frontal lobe of the cortex. Moderate expression is observed in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Defects in APP are a cause of autosomal dominant Alzheimer′s disease (AD). UP on intracerebroventricular injection of this antibody, A beta levels significantly decreased in the brain of APPPS1 mouse model of AD. A related antibody, Cat. No. MABN380, Anti-APP-C99, clone mC99(70-80) was raised against a slightly different region of the same APP-C99 protein.

免疫原

Recombinant protein corresponding to Human APP-C99.

应用

Research Category
Neuroscience
Anti-APP-C99 Antibody, clone mC99(1-7) is a highly specific mouse monoclonal antibody, that targets APP & has been tested in western blotting, IP, ICC & IHC.
Immunoprecipitation Analysis: A representative lot immunoprecipitated APP-C99 in HEK APPSwe cell lysate (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).

Immunohistochemistry Analysis: A representative lot detected APP-C99 in APPS1 mice and in human Alzheimer brain tissues
(Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).

Immunofluoresence Anlaysis: A representative lot detected APP-C99 in HEK APPSwe cells (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).
Research Sub Category
MMPs & TIMPs

质量

Evaluated by Western Blotting in DAPT treated HEK293 cell lysate.

Western Blotting Anlaysis: A 1:2,000 dilution of this antibody detected APP-C99 in 10 ?g of DAPT treated HEK293 cell lysate.

目标描述

~95 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

外形

Unpurified
Mouse monoclonal IgGκ ascites

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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