Anti-Apelin receptor Antibody

半岛bd体育手机客户端 说明

一般描述

The Apelin receptor (APJ receptor) is a Gi/o-coupled protein with significant sequence homology to the angiotensin II type 1 (AT1) receptor. It is activated by apelin peptides (12, 17, or 36 amino acids). The effects of the apelin receptor are mediated mainly through the activation of protein kinase C and calcium signaling. The apelin receptor has been detected in the brain, heart, spleen, thymus, ovary, small intestine, and colon. Several studies have suggested that it is involved in regulating cardiovascular function and glucose homeostasis.

特异性

This antibody recognizes Apelin receptor at the cytoplasmic domain.

免疫原

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Apelin receptor.
Epitope: Cytoplasmic domain

应用

Western Blot Analysis: 0.5 ?g/mL from a representative lot detected Apelin receptor on 10 ?g of human cerebellum tissue lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Apelin receptor in several regions of mouse brain sections.
This Anti-Apelin receptor Antibody is validated for use in Western Blotting, IHC(P) for the detection of Apelin receptor.
Research Category
Stem Cell Research

质量

Evaluated by Western Blot in mouse cerebellum tissue lysate.

Western Blot Analysis: 0.5 ?g/mL of this antibody detected Apelin receptor on 10 ?g of mouse cerebellum tissue lysate.

目标描述

~62 kDa observed. The calculated molecular weight of this protein is 42 kDa, but can be observed at ~62 kDa due to high glycosylation. (Medhurst, A., et al (2003). Journal of Neurochemistry. 84:1162–1172.)

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Mouse Cerebellum Tissue Lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human, mouse
species reactivity (predicted by homology)	rat (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
NCBI登记号	Np_005152
UniProt登记号	P35414
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable