Anti-ALS2 (N-terminal region) antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

Amyotrophic lateral sclerosis (ALS)2 gene codes for 184 kDa alsin protein. Alsin is characterized with the three putative guanine exchange factor (GEF) domains such as, RCC1-like domain (RLD), the Dbl and pleckstrin homology (DH/PH) domain, and a vacuolar protein sorting 9 (VPS9) domain. In addition, ALS2 also contains eight consecutive membrane occupation and recognition nexus (MORN) motifs. ALS2 mRNA is ubiquitously expressed in the CNS and non-neuronal tissues, with the highest expression in the cerebellum and kidney.

特异性

Anti-ALS2 (N-terminal region) specifically recognizes human, rat, and mouse ALS2.

应用

Anti-ALS2 (N-terminal region) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

生化/生理作用

Amyotrophic lateral sclerosis (ALS)2 has been shown to mediate the activation of Rab5 and Rac1/PACK1. In the nervous system, ALS2 is preferentially associated with the cytoplasmic side of endosome membrane, it modulates endosome membrane trafficking and promotes neurite growth in neuronal cultures. Loss-of function mutations in the ALS2 gene leads to the development of recessive motor neuron diseases, including forms of ALS (ALS2), juvenile PLS (PLSJ) and hereditary spastic paraplegia (HSP).

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

储存及稳定性

For continuous use, store at 2–8 ℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen ~185 kDa
包装	antibody small pack of 25 μL
浓度	~1.5 mg/mL
technique(s)	indirect immunofluorescence: 2-4 μg/mL using NIH3T3 cells western blot: 1.5-3.0 μg/mL using HEK-293T cell lysate over expressing human ALS2, and rat cerebellum extract (S1 fraction).
运输	dry ice
储存温度	?20℃
Gene Information	mouse ... Als2(74018) rat ... Als2(363235)

安全信息

储存分类代码	12 - Non Combustible Liquids
闪点(F)	Not applicable
闪点(C)	Not applicable